A
-
Allele
Independence of color intensity variation in red flesh apples from the number of repeat units in promoter region of the MdMYB10 gene as an allele to MdMYB1 and MdMYBA [Volume 10, Issue 3, 2012, Pages 153-160]
-
Antip53 antibody
Production and evaluation of polyclonal rabbit antihuman p53 antibody using bacterially expressed glutathione S-transferase-p53 fusion protein [Volume 10, Issue 4, 2012, Pages 240-248]
-
Arabidopsis
Functional analysis of Glycin Rich- RNA Binding protein, a suppressor of Trehalose-6-Phosphate mediating growth arrest in Arabidopsis thaliana [Volume 10, Issue 1, 2012, Pages 22-31]
-
Artemin
Identification of RNA-binding sites in artemin based on docking energy landscapes and molecular dynamics simulation [Volume 10, Issue 1, 2012, Pages 8-15]
B
-
Bacillus clausii
In silico genome-wide screening for TnrA-regulated genes of Bacillus clausii [Volume 10, Issue 1, 2012, Pages 62-66]
-
Bacillus megaterium
Comparative proteomics analysis of a novel γ-radiationresistant bacterium wild-type Bacillus megaterium strain WHO DQ973298 recovering from 5 KGy γ-irradiation [Volume 10, Issue 2, 2012, Pages 96-105]
-
Bacillus thermocatenulatus Lipase
Periplasmic expression of Bacillus thermocatenulatus lipase in Escherichia coli in presence of different signal sequences [Volume 10, Issue 4, 2012, Pages 255-262]
-
Batch process
Comparison between batch and fed-batch production of rhamnolipid by Pseudomonas aeruginosa [Volume 10, Issue 4, 2012, Pages 263-269]
-
Beta toxin
In silico fusion of epsilon and beta toxin genes of Clostridium perfringens types D and B [Volume 10, Issue 1, 2012, Pages 55-61]
-
Biochemical analysis
Factors influencing in vitro plant regeneration of Liquorice (Glycyrrhiza glabra L.) [Volume 10, Issue 3, 2012, Pages 161-167]
-
Bioinformatics
Proteome analysis of Cryptosporidium parvum and C. hominis using two-dimentional electrophoresis, image analysis and tandem mass spectrometry [Volume 10, Issue 3, 2012, Pages 198-207]
-
Biomaterials
Synthesis and characterization of fiber reinforced polymer scaffolds based on natural fibers and polymer for bone tissue engineering application [Volume 10, Issue 3, 2012, Pages 184-190]
-
Bone tissue engineering
Synthesis and characterization of fiber reinforced polymer scaffolds based on natural fibers and polymer for bone tissue engineering application [Volume 10, Issue 3, 2012, Pages 184-190]
-
Breeder hens
Association of prolactin and prolactin receptor gene polymorphisms with economic traits in breeder hens of indigenous chickens of Mazandaran province [Volume 10, Issue 2, 2012, Pages 129-135]
C
-
Calpastatin
Genetic variability of calpastatin and calpain genes in Iranian Zel sheep using PCR-RFLP and PCR-SSCP methods [Volume 10, Issue 2, 2012, Pages 136-139]
-
Canola
Heterologous expression of the Secale cereal thaumatinlike protein in transgenic canola plants enhances resistance to stem rot disease [Volume 10, Issue 2, 2012, Pages 87-95]
-
Caspian pony
Molecular characterization a Salmonella Typhimurium isolate from Caspian pony [Volume 10, Issue 1, 2012, Pages 49-54]
-
Characterization
Purification and characterization of a thermostable neutrophilic metalloprotease from Pseudomonas sp. DR89 [Volume 10, Issue 2, 2012, Pages 120-128]
-
Characterization
Isolation, purification and characterization of proline dehydrogenase from a Pseudomonas putida POS-F84 isolate [Volume 10, Issue 2, 2012, Pages 111-119]
-
Cladogram tree
Signal processing approaches as novel tools for the clustering of N-acetyl-β-D-glucosaminidases [Volume 10, Issue 3, 2012, Pages 175-183]
-
Cloning
Cloning, expression, purification and immunoreactivity analysis of gag derived protein p17 from HIV-1 CRF35 in fusion with thioredoxin from human subjects [Volume 10, Issue 4, 2012, Pages 249-254]
-
Cloning
Construction of a synthetic vector for preparation of a 100 base pair DNA ladder [Volume 10, Issue 2, 2012, Pages 106-110]
-
Clostridium perfringens
In silico fusion of epsilon and beta toxin genes of Clostridium perfringens types D and B [Volume 10, Issue 1, 2012, Pages 55-61]
-
Color intensity
Independence of color intensity variation in red flesh apples from the number of repeat units in promoter region of the MdMYB10 gene as an allele to MdMYB1 and MdMYBA [Volume 10, Issue 3, 2012, Pages 153-160]
-
Comparative proteomics
Proteome analysis of Cryptosporidium parvum and C. hominis using two-dimentional electrophoresis, image analysis and tandem mass spectrometry [Volume 10, Issue 3, 2012, Pages 198-207]
-
Correlation
Signal processing approaches as novel tools for the clustering of N-acetyl-β-D-glucosaminidases [Volume 10, Issue 3, 2012, Pages 175-183]
-
Cryptosporidium
Proteome analysis of Cryptosporidium parvum and C. hominis using two-dimentional electrophoresis, image analysis and tandem mass spectrometry [Volume 10, Issue 3, 2012, Pages 198-207]
D
-
DNA marker
Construction of a synthetic vector for preparation of a 100 base pair DNA ladder [Volume 10, Issue 2, 2012, Pages 106-110]
-
Drought stress
Improving rice (Oryza sativa L.) drought tolerance by suppressing a NF-YA transcription factor [Volume 10, Issue 1, 2012, Pages 40-48]
-
Drug metabolism
Expression of cytochrome P450 and glutathione S-transferase in human bone marrow mesenchymal stem cells [Volume 10, Issue 4, 2012, Pages 270-274]
-
Drug resistance
Drug resistance profile and subtyping of HIV-1 RT gene among Iranian under-treatment patients [Volume 10, Issue 1, 2012, Pages 1-7]
E
-
Enumeration
Suitability of MRS-bile agar for the selective enumeration of mixed probiotic bacteria in presence of mesophilic lactic acid cultures and yoghurt bacteria [Volume 10, Issue 1, 2012, Pages 16-21]
-
Environmental
Microbial cell surface display; its medical and environmental applications [Volume 10, Issue 4, 2012, Pages 231-239]
-
Enzyme clustering
Signal processing approaches as novel tools for the clustering of N-acetyl-β-D-glucosaminidases [Volume 10, Issue 3, 2012, Pages 175-183]
-
Escherichia coli
Optimization of secretory expression of recombinant hGM-CSF in high cell density cultivation of recombinant Escherichia coli using Taguchi statistical method [Volume 10, Issue 4, 2012, Pages 275-280]
-
Essential oil
Gene expression and activity of phenyl alanine amonialyase and essential oil composition of Ocimum basilicum L. at different growth stages [Volume 10, Issue 1, 2012, Pages 32-39]
-
Ethanol production
Process optimization for ethanol production from very high gravity (VHG) finger millet medium using response surface methodology [Volume 10, Issue 3, 2012, Pages 168-174]
-
Explanting season
Factors influencing in vitro plant regeneration of Liquorice (Glycyrrhiza glabra L.) [Volume 10, Issue 3, 2012, Pages 161-167]
-
Expression
Tissue specific expression of human calcitonin gene in potato tubers by an organ specific promoter [Volume 10, Issue 2, 2012, Pages 79-86]
F
-
Fat digestibility
The effects of non-starch polysaccharides content of wheat and xylanase supplementation on the intestinal amylase, aminopeptidase and lipase activities, ileal viscosity and fat digestibility in layer diet [Volume 10, Issue 3, 2012, Pages 208-214]
-
Fed-batch
Optimization of secretory expression of recombinant hGM-CSF in high cell density cultivation of recombinant Escherichia coli using Taguchi statistical method [Volume 10, Issue 4, 2012, Pages 275-280]
-
Fed batch process
Comparison between batch and fed-batch production of rhamnolipid by Pseudomonas aeruginosa [Volume 10, Issue 4, 2012, Pages 263-269]
-
Fiber reinforced polymer (FRP) composites
Synthesis and characterization of fiber reinforced polymer scaffolds based on natural fibers and polymer for bone tissue engineering application [Volume 10, Issue 3, 2012, Pages 184-190]
-
Finger millet medium
Process optimization for ethanol production from very high gravity (VHG) finger millet medium using response surface methodology [Volume 10, Issue 3, 2012, Pages 168-174]
-
Fusion
In silico fusion of epsilon and beta toxin genes of Clostridium perfringens types D and B [Volume 10, Issue 1, 2012, Pages 55-61]
-
Fusion protein
Cloning, expression, purification and immunoreactivity analysis of gag derived protein p17 from HIV-1 CRF35 in fusion with thioredoxin from human subjects [Volume 10, Issue 4, 2012, Pages 249-254]
G
-
Gene regulation
In silico genome-wide screening for TnrA-regulated genes of Bacillus clausii [Volume 10, Issue 1, 2012, Pages 62-66]
-
Genetics
The association between selected RANKL gene polymorphisms and chronic/aggressive periodontitis in Iranian subjects [Volume 10, Issue 3, 2012, Pages 215-220]
-
Glutathione S-transferases, cytochrome P450
Expression of cytochrome P450 and glutathione S-transferase in human bone marrow mesenchymal stem cells [Volume 10, Issue 4, 2012, Pages 270-274]
-
Green synthesis
Comparative study on silver nanoparticles properties produced by green methods [Volume 10, Issue 3, 2012, Pages 191-197]
-
GR-RBP2
Functional analysis of Glycin Rich- RNA Binding protein, a suppressor of Trehalose-6-Phosphate mediating growth arrest in Arabidopsis thaliana [Volume 10, Issue 1, 2012, Pages 22-31]
-
GST-p53
Production and evaluation of polyclonal rabbit antihuman p53 antibody using bacterially expressed glutathione S-transferase-p53 fusion protein [Volume 10, Issue 4, 2012, Pages 240-248]
-
Gus fusion
Functional analysis of Glycin Rich- RNA Binding protein, a suppressor of Trehalose-6-Phosphate mediating growth arrest in Arabidopsis thaliana [Volume 10, Issue 1, 2012, Pages 22-31]
H
-
High cell density cultivation
Optimization of secretory expression of recombinant hGM-CSF in high cell density cultivation of recombinant Escherichia coli using Taguchi statistical method [Volume 10, Issue 4, 2012, Pages 275-280]
-
HIV-1
Drug resistance profile and subtyping of HIV-1 RT gene among Iranian under-treatment patients [Volume 10, Issue 1, 2012, Pages 1-7]
-
HIV-1 CRF35_AD
Cloning, expression, purification and immunoreactivity analysis of gag derived protein p17 from HIV-1 CRF35 in fusion with thioredoxin from human subjects [Volume 10, Issue 4, 2012, Pages 249-254]
-
Human bone marrow mesenchymal stem cells
Expression of cytochrome P450 and glutathione S-transferase in human bone marrow mesenchymal stem cells [Volume 10, Issue 4, 2012, Pages 270-274]
-
Human calcitonin
Tissue specific expression of human calcitonin gene in potato tubers by an organ specific promoter [Volume 10, Issue 2, 2012, Pages 79-86]
-
Human granulocyte macrophage colony stimulating factor
Optimization of secretory expression of recombinant hGM-CSF in high cell density cultivation of recombinant Escherichia coli using Taguchi statistical method [Volume 10, Issue 4, 2012, Pages 275-280]
I
-
Immunoassays
Production and evaluation of polyclonal rabbit antihuman p53 antibody using bacterially expressed glutathione S-transferase-p53 fusion protein [Volume 10, Issue 4, 2012, Pages 240-248]
-
Inclusion bodies
Periplasmic expression of Bacillus thermocatenulatus lipase in Escherichia coli in presence of different signal sequences [Volume 10, Issue 4, 2012, Pages 255-262]
-
In Silico
In silico fusion of epsilon and beta toxin genes of Clostridium perfringens types D and B [Volume 10, Issue 1, 2012, Pages 55-61]
-
Intestinal enzymes
The effects of non-starch polysaccharides content of wheat and xylanase supplementation on the intestinal amylase, aminopeptidase and lipase activities, ileal viscosity and fat digestibility in layer diet [Volume 10, Issue 3, 2012, Pages 208-214]
-
Iran
Drug resistance profile and subtyping of HIV-1 RT gene among Iranian under-treatment patients [Volume 10, Issue 1, 2012, Pages 1-7]
L
-
Laying hen
The effects of non-starch polysaccharides content of wheat and xylanase supplementation on the intestinal amylase, aminopeptidase and lipase activities, ileal viscosity and fat digestibility in layer diet [Volume 10, Issue 3, 2012, Pages 208-214]
-
Liquorice
Factors influencing in vitro plant regeneration of Liquorice (Glycyrrhiza glabra L.) [Volume 10, Issue 3, 2012, Pages 161-167]
M
-
Malus×domestica
Independence of color intensity variation in red flesh apples from the number of repeat units in promoter region of the MdMYB10 gene as an allele to MdMYB1 and MdMYBA [Volume 10, Issue 3, 2012, Pages 153-160]
-
Mass spectrometry
Proteome analysis of Cryptosporidium parvum and C. hominis using two-dimentional electrophoresis, image analysis and tandem mass spectrometry [Volume 10, Issue 3, 2012, Pages 198-207]
-
MdMYB
Independence of color intensity variation in red flesh apples from the number of repeat units in promoter region of the MdMYB10 gene as an allele to MdMYB1 and MdMYBA [Volume 10, Issue 3, 2012, Pages 153-160]
-
Medical
Microbial cell surface display; its medical and environmental applications [Volume 10, Issue 4, 2012, Pages 231-239]
-
Methyl chavicol
Gene expression and activity of phenyl alanine amonialyase and essential oil composition of Ocimum basilicum L. at different growth stages [Volume 10, Issue 1, 2012, Pages 32-39]
-
Microbial Surface Display
Microbial cell surface display; its medical and environmental applications [Volume 10, Issue 4, 2012, Pages 231-239]
-
Microbial synthesis
Comparative study on silver nanoparticles properties produced by green methods [Volume 10, Issue 3, 2012, Pages 191-197]
-
Molecular Dynamics Simulation
Identification of RNA-binding sites in artemin based on docking energy landscapes and molecular dynamics simulation [Volume 10, Issue 1, 2012, Pages 8-15]
-
Mother wavelet
Signal processing approaches as novel tools for the clustering of N-acetyl-β-D-glucosaminidases [Volume 10, Issue 3, 2012, Pages 175-183]
-
MRS-bile agar
Suitability of MRS-bile agar for the selective enumeration of mixed probiotic bacteria in presence of mesophilic lactic acid cultures and yoghurt bacteria [Volume 10, Issue 1, 2012, Pages 16-21]
-
Multiple shoots
Factors influencing in vitro plant regeneration of Liquorice (Glycyrrhiza glabra L.) [Volume 10, Issue 3, 2012, Pages 161-167]
-
Multiplex PCR
Molecular characterization a Salmonella Typhimurium isolate from Caspian pony [Volume 10, Issue 1, 2012, Pages 49-54]
N
-
Neutrophilic protease
Purification and characterization of a thermostable neutrophilic metalloprotease from Pseudomonas sp. DR89 [Volume 10, Issue 2, 2012, Pages 120-128]
-
Nodal segments
Factors influencing in vitro plant regeneration of Liquorice (Glycyrrhiza glabra L.) [Volume 10, Issue 3, 2012, Pages 161-167]
-
Nuclear Factor Y
Improving rice (Oryza sativa L.) drought tolerance by suppressing a NF-YA transcription factor [Volume 10, Issue 1, 2012, Pages 40-48]
O
-
Ocimum basilicum L
Gene expression and activity of phenyl alanine amonialyase and essential oil composition of Ocimum basilicum L. at different growth stages [Volume 10, Issue 1, 2012, Pages 32-39]
P
-
Periodontitis
The association between selected RANKL gene polymorphisms and chronic/aggressive periodontitis in Iranian subjects [Volume 10, Issue 3, 2012, Pages 215-220]
-
Periplasmic space
Periplasmic expression of Bacillus thermocatenulatus lipase in Escherichia coli in presence of different signal sequences [Volume 10, Issue 4, 2012, Pages 255-262]
-
PFGE
Molecular characterization a Salmonella Typhimurium isolate from Caspian pony [Volume 10, Issue 1, 2012, Pages 49-54]
-
Photoperiod
Factors influencing in vitro plant regeneration of Liquorice (Glycyrrhiza glabra L.) [Volume 10, Issue 3, 2012, Pages 161-167]
-
Phylogeny
Drug resistance profile and subtyping of HIV-1 RT gene among Iranian under-treatment patients [Volume 10, Issue 1, 2012, Pages 1-7]
-
Plasmid
Construction of a synthetic vector for preparation of a 100 base pair DNA ladder [Volume 10, Issue 2, 2012, Pages 106-110]
-
Polymorphism
Association of prolactin and prolactin receptor gene polymorphisms with economic traits in breeder hens of indigenous chickens of Mazandaran province [Volume 10, Issue 2, 2012, Pages 129-135]
-
Polymorphism
Genetic variability of calpastatin and calpain genes in Iranian Zel sheep using PCR-RFLP and PCR-SSCP methods [Volume 10, Issue 2, 2012, Pages 136-139]
-
Polysaccharide method
Comparative study on silver nanoparticles properties produced by green methods [Volume 10, Issue 3, 2012, Pages 191-197]
-
Probiotic
Suitability of MRS-bile agar for the selective enumeration of mixed probiotic bacteria in presence of mesophilic lactic acid cultures and yoghurt bacteria [Volume 10, Issue 1, 2012, Pages 16-21]
-
Pseudomonas aeruginosa
Comparison between batch and fed-batch production of rhamnolipid by Pseudomonas aeruginosa [Volume 10, Issue 4, 2012, Pages 263-269]
-
Pseudomonas putida
Isolation, purification and characterization of proline dehydrogenase from a Pseudomonas putida POS-F84 isolate [Volume 10, Issue 2, 2012, Pages 111-119]
-
Purification
Purification and characterization of a thermostable neutrophilic metalloprotease from Pseudomonas sp. DR89 [Volume 10, Issue 2, 2012, Pages 120-128]
-
Purification
Isolation, purification and characterization of proline dehydrogenase from a Pseudomonas putida POS-F84 isolate [Volume 10, Issue 2, 2012, Pages 111-119]
-
Purification
Optimization of secretory expression of recombinant hGM-CSF in high cell density cultivation of recombinant Escherichia coli using Taguchi statistical method [Volume 10, Issue 4, 2012, Pages 275-280]
R
-
RANKL
The association between selected RANKL gene polymorphisms and chronic/aggressive periodontitis in Iranian subjects [Volume 10, Issue 3, 2012, Pages 215-220]
-
Recombinant protein
Cloning, expression, purification and immunoreactivity analysis of gag derived protein p17 from HIV-1 CRF35 in fusion with thioredoxin from human subjects [Volume 10, Issue 4, 2012, Pages 249-254]
-
Red flesh
Independence of color intensity variation in red flesh apples from the number of repeat units in promoter region of the MdMYB10 gene as an allele to MdMYB1 and MdMYBA [Volume 10, Issue 3, 2012, Pages 153-160]
-
Response Surface Methodology
Process optimization for ethanol production from very high gravity (VHG) finger millet medium using response surface methodology [Volume 10, Issue 3, 2012, Pages 168-174]
-
Rhamnolipid
Comparison between batch and fed-batch production of rhamnolipid by Pseudomonas aeruginosa [Volume 10, Issue 4, 2012, Pages 263-269]
-
Rice (Oryza sativa L.)
Improving rice (Oryza sativa L.) drought tolerance by suppressing a NF-YA transcription factor [Volume 10, Issue 1, 2012, Pages 40-48]
-
RNA-protecting role
Identification of RNA-binding sites in artemin based on docking energy landscapes and molecular dynamics simulation [Volume 10, Issue 1, 2012, Pages 8-15]
S
-
Salmonella Typhimurium
Molecular characterization a Salmonella Typhimurium isolate from Caspian pony [Volume 10, Issue 1, 2012, Pages 49-54]
-
Sclerotinia sclerotiorum
Heterologous expression of the Secale cereal thaumatinlike protein in transgenic canola plants enhances resistance to stem rot disease [Volume 10, Issue 2, 2012, Pages 87-95]
-
Secale cereal
Heterologous expression of the Secale cereal thaumatinlike protein in transgenic canola plants enhances resistance to stem rot disease [Volume 10, Issue 2, 2012, Pages 87-95]
-
Sheep
Genetic variability of calpastatin and calpain genes in Iranian Zel sheep using PCR-RFLP and PCR-SSCP methods [Volume 10, Issue 2, 2012, Pages 136-139]
-
Signal processing
Signal processing approaches as novel tools for the clustering of N-acetyl-β-D-glucosaminidases [Volume 10, Issue 3, 2012, Pages 175-183]
-
Silk
Synthesis and characterization of fiber reinforced polymer scaffolds based on natural fibers and polymer for bone tissue engineering application [Volume 10, Issue 3, 2012, Pages 184-190]
-
Silver nanoparticles
Comparative study on silver nanoparticles properties produced by green methods [Volume 10, Issue 3, 2012, Pages 191-197]
-
Single nucleotide polymorphism
The association between selected RANKL gene polymorphisms and chronic/aggressive periodontitis in Iranian subjects [Volume 10, Issue 3, 2012, Pages 215-220]
-
Subculture time
Factors influencing in vitro plant regeneration of Liquorice (Glycyrrhiza glabra L.) [Volume 10, Issue 3, 2012, Pages 161-167]
T
-
Tandem mass spectrometry
Periplasmic expression of Bacillus thermocatenulatus lipase in Escherichia coli in presence of different signal sequences [Volume 10, Issue 4, 2012, Pages 255-262]
-
Tandem repeats
Independence of color intensity variation in red flesh apples from the number of repeat units in promoter region of the MdMYB10 gene as an allele to MdMYB1 and MdMYBA [Volume 10, Issue 3, 2012, Pages 153-160]
-
Taptaged
Functional analysis of Glycin Rich- RNA Binding protein, a suppressor of Trehalose-6-Phosphate mediating growth arrest in Arabidopsis thaliana [Volume 10, Issue 1, 2012, Pages 22-31]
-
Thaumatin-like proteins
Heterologous expression of the Secale cereal thaumatinlike protein in transgenic canola plants enhances resistance to stem rot disease [Volume 10, Issue 2, 2012, Pages 87-95]
-
Thermostability
Purification and characterization of a thermostable neutrophilic metalloprotease from Pseudomonas sp. DR89 [Volume 10, Issue 2, 2012, Pages 120-128]
-
TnrA
In silico genome-wide screening for TnrA-regulated genes of Bacillus clausii [Volume 10, Issue 1, 2012, Pages 62-66]
-
Tollens method
Comparative study on silver nanoparticles properties produced by green methods [Volume 10, Issue 3, 2012, Pages 191-197]
-
Transgenic plant
Heterologous expression of the Secale cereal thaumatinlike protein in transgenic canola plants enhances resistance to stem rot disease [Volume 10, Issue 2, 2012, Pages 87-95]
-
Transportation
Molecular characterization a Salmonella Typhimurium isolate from Caspian pony [Volume 10, Issue 1, 2012, Pages 49-54]
-
Trehalose
Functional analysis of Glycin Rich- RNA Binding protein, a suppressor of Trehalose-6-Phosphate mediating growth arrest in Arabidopsis thaliana [Volume 10, Issue 1, 2012, Pages 22-31]
-
Tumor
Production and evaluation of polyclonal rabbit antihuman p53 antibody using bacterially expressed glutathione S-transferase-p53 fusion protein [Volume 10, Issue 4, 2012, Pages 240-248]
-
Two-dimensional gel electrophoresis
Comparative proteomics analysis of a novel γ-radiationresistant bacterium wild-type Bacillus megaterium strain WHO DQ973298 recovering from 5 KGy γ-irradiation [Volume 10, Issue 2, 2012, Pages 96-105]
V
-
Very high gravity (VHG) fermentation
Process optimization for ethanol production from very high gravity (VHG) finger millet medium using response surface methodology [Volume 10, Issue 3, 2012, Pages 168-174]
W
-
Wheat
The effects of non-starch polysaccharides content of wheat and xylanase supplementation on the intestinal amylase, aminopeptidase and lipase activities, ileal viscosity and fat digestibility in layer diet [Volume 10, Issue 3, 2012, Pages 208-214]
X
-
Xylanase
The effects of non-starch polysaccharides content of wheat and xylanase supplementation on the intestinal amylase, aminopeptidase and lipase activities, ileal viscosity and fat digestibility in layer diet [Volume 10, Issue 3, 2012, Pages 208-214]
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