In silico fusion of epsilon and beta toxin genes of Clostridium perfringens types D and B

Document Type: Short Paper

Authors

1 1Department of Molecular Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Karaj Highway, P.O. Box 14965/161, I.R. Iran.

2 Department of Anaerobic Bacterial Vaccine Research and Production, Razi Vaccine and Serum Research Institute, Karaj, I.R. Iran 3Department of Genomix and Genetic Engineering, Razi Vaccine and Serum Research Institute, Karaj, I.R. Iran.

3 Department of Genomix and Genetic Engineering, Razi Vaccine and Serum Research Institute, Karaj, I.R. Iran.

4 Department of Molecular Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Karaj Highway, P.O. Box 14965/161, I.R. Iran.

Abstract

Fusion protein technology represents the strategy to achieve rapid, efficient, and cost-effective protein
expression. Epsilon and Beta toxins are the most potent Clostridial toxins and cause disease in animals.
This study describes in silico fusion of Clostridium perfringens types D and B epsilon and beta toxin genes
that was used for cloning in E.coli. The etx and cpb genes were retrieved from the GenBank and a fusion
gene was designed to produce a chimeric fusion protein. Secondary and tertiary structures and specificities
of fusion protein were determined by online software. Results showed that the designed fusion gene construction is suitable for chimeric fusion protein expression.

Keywords