Department of Chemical Engineering, Science and Research Branch, Islamic Azad University, P.O. Box 1477893855, Tehran, I.R. Iran.
Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14965/161, Tehran, I.R. Iran.
Human granulocyte macrophage colony stimulating factor (hGM-CSF) has many therapeutic applications.
In this study, in order to verify the purification process, the effect of carbon source, IPTG concentration and
post-induction time on the secretion of recombinant hGM-CSF into the culture medium by recombinant
Escherichia coli during high cell density cultivation were evaluated by using the Taguchi statistical method. The results indicated that glucose, 1mM IPTG and a time of 6 h post-induction, represented optimum conditions. The secreted hGM-CSF, overall volumetric productivity and purified hGM-CSF were 373 mg/l, 18 mg/l/h and 63 mg/l, respectively.