Document Type: Brief Report
Laboratory for Molecular Genetics and Animal Biotechnology, Department of Animal Science, Faculty of Animal Science and Fisheries, Sari Agricultural Science and Natural Resources University, P.O. Box 578, Sari, I.R. Iran.
Polymorphisms in 5’-flanking region of prolactin (PRL), exon 2 and exon 5 of prolactin receptor (PRLR) genes
and its association with growth and egg traits were examined in breeder hens of Mazandaran native fowls
breeding station. A single nucleotide polymorphism at site C-2402T and a 24 bp nucleotide sequence insertion
at situation -382 in 5’-flanking regions of PRL gene were identified. PCR amplification together with
Restriction Fragment Length Polymorphism (RFLP) and direct agarose gel electrophoresis were used to identify different genotypes at C-2402T and a 24 bp indel (insertion-deletion) at the site of -358 of PRL gene, respectively. Nucleotide substitution of C to T and a 24 nucleotides insertion (I) or deletion (D) in promoter
region of PRL gene resulted in three genotypes with the frequency of CC (0.10), CT (0.84), TT (0.06)
and II (0.39), ID (0.40), DD (0.21), respectively. There were no heterozygous females and only two genotypes A/A (0.54), B/B (0.46) and AA (0.72), BB (0.28) were identified in exon 2 and exon 5 of PRLR gene
using PCR-Single Strand Conformation Polymorphism (PCR-SSCP) and PCR-RFLP analyses, respectively. A
novel mutation consists of a BamHI restriction site found in the exon 5 of PRLR gene. The results showed
significant association between SNP in exon 2 with body weight at hatch, age at sexual maturity, and
between SNP in exon 5 and egg number. Individuals with AA genotype produced higher eggs than BB genotype (P<0.05). These results showed that the PRLR locus can be considered as a major gene that may
influence the production traits in chicken.