In silico genome-wide screening for TnrA-regulated genes of Bacillus clausii

Document Type: Brief Report

Authors

1 1Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14965-161, Tehran, I.R. Iran.

2 Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), P.O. Box 15815-3538, Tehran, I.R. Iran.

3 Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14965-161, Tehran, I.R. Iran.

Abstract

Bacillus clausii TnrA transcription factor is required for global nitrogen regulation. In order to obtain an
overview of gene regulation by TnrA in B. clausii KSMK16, the entire genome of B. clausii was screened for
the consensus sequence, 5’-TGTNAN7TNACA-3’ known as the TnrA box, and 13 transcription units were
found containing a putative TnrA box. The TnrA targets identified in this study were tnrA, glnA, nrgA,
nasFDEB, puc genes, licT, the two operons of the oligopeptide ABC transporter, lytR, transcriptional regulator
of the Lrp/AsnC family, sodium-dependent transporter of SNF family, hyu genes and a biochemically
uncharacterized protein.

Keywords