Document Type: Research Paper
Department of Biology, Faculty of Science, Golestan University, P.O.Box 155, Gorgan, I.R. Iran.
Department of Molecular Plant Biology, Institute of Environmental Biology, Utrecht University, Padualaan 8, 3584 CH, The Netherlands.
Metabolism of the alpha-1,1 glucose disaccharide, trehalose, is indispensable in plants. In the Murashige
and Skoog (MS) medium, trehalose inhibits plant growth and allocation of carbon to roots. A suppressor
of trehalose-6-phosphate (T6P) mediated growth arrest, GR-RBP2, is characterized in more detail.
Phylogenetic analysis revealed that GR-RBP2 is a protein of likely prokaryotic origin. A knockout mutant
of GR-RBP2 was identified in the T-DNA insertion line SALK-059714, yet plants of this line were not altered
with regard to growth on different carbon sources and on trehalose compared to WT. GUS expression analysis
showed that GR-RBP2 was detected in adult leaves, flowers and siliques. Expression was particularly
high in root tips. GR-RBP2 expression also is insensitive to 100 mM trehalose. TAP-tagged versions
of this protein showed that GR-RBP2 is part of a protein complex in planta.