Guide for Authors


This Journal accepts only manuscripts through the online submission system. It is easy to use and easy to track, thus by conducting all steps electronically; your submission will be expedient. Contributors can submit their manuscripts via our website. Once you submit an article, it will be forwarded to one of the editors and afterwards to at least two peer-reviewers. The author will be notified of the submission process by means of E-mail. Simultaneously, "Copyright Transfer Letter" form should be filled and uploaded on the website.


This letter should be uploaded online as a word file. The author should state that the manuscript has not been and will not be published or submitted elsewhere. The financial disclosure should be declared. The letter must include a statement declaring that the study complies with current ethical considerations. Authors should declare that all figures of their manuscripts are original. Authors reporting experimental studies on human subjects must include a statement of assurance in the Materials and Methods section of the manuscript reading that: (1) informed consent was obtained from each patient included in the study and (2) the study protocol conforms to the ethical guidelines of the 1975 Declaration of Helsinki.


Manuscripts should be in Word Document (Microsoft Word 97, 2003, 2007, 2010, 2013) in English and should follow the style of the Uniform Requirements for Manuscripts Submitted to Biomedical Journals, a copy of which can be found at

Note 1. Submit your full manuscript with figures and tables. Figures and tables should be sent as separate files. 



• Case Report: 1500 - 2000 words (Maximum number of references is 15, Maximum Number of illustrations/Tables is 2)

• Brief Report: 1500 - 2000 words (Maximum number of references is 15, Maximum Number of illustrations/Tables is 2)

• Research Articles: 2500 - 3500 words (Maximum number of references is 40, Maximum Number of illustrations/Tables is 5)

• Review Articles: 3500 - 4000 words (Maximum number of references is 60, Maximum Number of illustrations/Tables is 5)

• Systematic reviews: 3500-4000 words (Maximum number of references is 60, Maximum Number of illustrations/Tables is 5)

• Letter: Less than 1500 words. A maximum of three authors and 10 references are allowed. Neither tables nor figures are allowed.

• Figure legend (including multiple figure parts): maximum 50 words

• Acknowledgments (not including research funding): 45-55 words.



Use the font Times New Roman size, Line spacing=Double.

  • Manuscript Main Title: Bold 16. The first letter should be Capital.
  • Affiliations: Bold 11. Numbers: Superscript 8
  • Corresponding Author: Italic, Superscript 8
  • Abstract: Regular 10
  • Main text: Regular 11
  • Main text titles: Regular 11. The first letter should be Capital.
  • Subtitles: Italic 11. The first letter should be Capital.


The title page should include:

• Title: Capitalize the initial of each word except prepositions with less than three letters.

• Author(s): full name of all authors should be mentioned.

• Affiliation: Author’s affiliation containing: Department, Faculty/School/Institute, University, City, and Country.

• Corresponding author: one of the authors (a Ph.D. holder) should be chosen. Address (Including P.O.BOX.), telephone and fax number and E-mail should be written.

Note 2. Email address of Corresponding Author should be Academic one.



[required for case reports, brief report/short communication, research articles, review articles]

• Abstract of brief report/short communication and research articles should be structured as below:

Background, Objectives, Materials and Methods, Results and Conclusions. A list of 3-6 keywords must be provided for indexing purposes. All keywords should be provided according to MeSH terms at: and should be alphabetically ordered.



Generally, includes the: Background, Objectives, Materials and Methods, Results, Discussion, Conclusions, Acknowledgments and References.

1. Background: This should summarize the rationale for the study.

2. Objectives: State the aims of the study.

3. Materials and Methods: This should include exact method or observation or experiment. If an apparatus is used, its manufacturer’s name and address should be given in parentheses. If a drug is used, its generic name, dose and route of administration must be given. Statistical method must be mentioned and specify any general computer program used.

  • For measurement units, use (L) for Liter, (mL) for milliliter.
  • For introducing units of measurement, use point (.) instead of fraction sign (/) (e.g. mg.L-1).
  • Consider a space between value and (ºC).
  • For time measurement use (h) for hour, (min) for minutes, (s) for seconds.
  • For centrifugation use (x g) instead of (rpm).

4. Results: Must be presented in the form of text, tables and illustrations. The contents of the tables should not be repeated in the text. Instead, a reference to the table number may be given. Long articles may need sub-headings within some sections (especially the Results and Discussion).

5. Discussion: This should emphasize the present findings and their differences or similarities with other work done in the field by other workers. Emphasize the new and important aspects of the study and the conclusions.

Note 3. Results and Discussion should come in separate sections.

6. Conclusion: This section provides a succinct explanation of the manuscript from the question of the research to the main finding of that.

• Acknowledgments: All contributors who do not meet the criteria for authorship should be covered in the acknowledgment section. Persons who provided technical help, writing assistance and should also be acknowledged.


Tables: All tables must be included at the end of the manuscript.

  • Tables in the word file should be separated by page break (each table on a separate page).
  • Each table should have borders with normal style without any colored row or column.
  • The style of table should be simple.
  • Each cell contains only one paragraph or one line.
  • Addressing tables in the text should be in bold.
  • Font of table should be Arial.


Figures: Figures must be submitted as separate files in the form of tif (.tif). The original data of the figure as excel file (.xls) and high resolution photo of gel (electrophoresis) should be sent, as well.

  • Colored figures are preferred (with no extra publication fee).
  • Font of figures should be Arial.
  • Addressing figures in the text should be in bold.
  • If a figure is constructed from more than one part, lable each part with bold capital letters such as A), B), C), … at top left corner
  • Resolution should be 300 dpi for a 3*2 inch figure.


Units, symbols, and abbreviations: Internationally accepted units (International System of Units), symbols, and abbreviations must be used. Abbreviations should be used sparingly and must be introduced in parentheses upon the first mention.

Drug names: Generic drug names must be used.



Note 4. All references that are referred in the main text should be arranged based on the End Note software. They also should be numbered based on their allocated numbers in the reference section.

Note 5. All References should have DOI. Journal names should be in abbreviation.

This Journal accepts references according to Vancouver style (with some minor changes) rules established by the International Committee of Medical Journal Editors. In the Vancouver system, the only indication required in the text of a paper is a number, allocated in ascending sequence, and presented in the text either in brackets. For example: “Recent randomized controlled trials in primary care showed benefits for patients with depression from increased telephone support, better cooperation between primary care and mental health professionals, and more systematic follow up (7).”If the same source is cited again later in the text, the same number is used once more. If multiple references are cited, use a hyphen to join an inclusive range of numbers thus: (2-5). Use commas without spaces to separate non-inclusive numbers in a multiple citation thus: (2-5, 7, 10).

• Books and Other Monographs

The details needed to construct a book reference are presented below. Each author’s surname followed by the initials (in the same order as they appear on the title page), a comma should separate each author’s name. Title of the book. Edition of the book if there has been more than one. Place of publication or town of origin followed by a colon, Publisher’s name, followed by a semi-colon, Year of publication. For example:

Ford MJ, Munro JF. Introduction to Clinical Examination. 7th ed. Edinburgh: Churchill Livingstone; 2000.

If only a part is cited, add the page numbers, and volume number in the case of multi-volume works, at the end of the reference. For example:

Meltzer PS, Kallioniemi A, Trent JM. Chromosome Alterations in Human Solid Tumors. In: Vogelstein B, Kinzler KW, editors. The Genetic Basis of Human Cancer. New York: McGraw-Hill; 2002. p. 93-113.

If you need to reference a book written by more than six authors, list the first six authors’ names followed by “et al”. For example:

Cohen J, Brown A, Smith B, Jones G, Murphy M, McKay P et al.

For standard journal article, list the first six authors followed by et al., paper title, journal title abbreviation (italic), year of publishing, volume number (bold), issue number in parentheses, page numbers and doi should also be presented. For example:

Meydani SN, Leka LS, Fine BC, Dallal GE, Keusch GT, Singh MF, et al. Vitamin E and Respiratory Tract Iinfections in Elderly Nursing Home Residents: a Randomized Controlled Trial. JAMA. 2004;292(7):828-836, doi: 10.1001/jama.292.7.828.

For all websites references you need to note the date that you accessed the information, and database name or web address (URL). For example:

Royal College of General Practitioners. The Primary Health Care Team.

RCGP website 2003 [cited 2004 Sep 22]; Available from: URL:



All submitted manuscripts are subject to peer review and editorial approval. Articles will be sent to at least 2 reviewers. Authors are usually notified within 1-2 months about the acceptability of their manuscript. Once receiving reviewers comments, authors are requested to send the revised article, and a copy of their reply to the reviewers, including the comment and explaining the replies to questions and changes made to the revised version within 48 hours.


Proofs will be sent to the corresponding author. Galley proofs should be returned within 48 hours from receipt.

Corresponding author is responsible for the accuracy of the manuscript content (including English writing and grammar).  



This Journal publishes Case Report, Brief Report/Short Communication, Research Articles, Review Articles, Systematic Review (Meta-Analysis), and Letter to the Editor

Case Report: A case report is a case study, case report, or other description of a case that should contain a structured/nonstructured abstract. Case reports should comprise sections including Introduction, Case Presentation, and Conclusions in Abstract, and Case Presentation, and Discussion in the text.

Brief Report/Short Communication: Short reports are usually a concise format used to report significant improvements to existing methods, a new practical application, or a new tool or resource. These need to be reported quickly as the need to communicate such findings is very high. Short communication should comprise sections including a structured abstract of 200 words maximum. A brief report should comprise sections of Introduction, Materials and Methods, Results and Discussion.

Research Articles: Research article or “Original Article” is a detailed study reporting original research conducted by the author. For the research article, traditional division into sections is required: Title, Keywords, Addresses and which author address for correspondence, Structured abstract, Background, Objectives, Materials and Methods, Results, Discussion, Conclusion (optional), Acknowledgements and References, Legends for Figures and Tables.

Review Articles: In a review article both abstract and text of the manuscript, include following items:

◊ Context: Include 1 or 2 sentences describing the clinical question or issue and its importance in clinical practice or public health.

◊ Evidence Acquisition: Describe the data sources used, including the search strategies, years searched, and other sources of material, such as subsequent reference searches of retrieved articles. Explain the methods used for quality assessment and the inclusion of relevant articles.

◊ Results: Address the major findings of the review of the clinical issue or topic in an evidence-based, objective, and balanced fashion, emphasizing the highest-quality evidence available.

◊ Conclusions: Clearly state the conclusions to answer the questions posed if applicable, basing the conclusions on available evidence, and emphasize how clinicians should apply current knowledge.

• Systematic Review (Meta-Analysis)

Authors should report systematic reviews in accordance with the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) statement. For Systematic Reviews, the abstract should be subdivided into the following sequential sections:

Context; Data Sources; Study Selection; Data Extraction; Results; Conclusions; and Keywords. Systematic reviews should be 3500-4000 words (abstracts to be structured as above). Consult: The Cochrane Reviewers’ Handbook Meta-analysis of observational studies.

(Review Paper" - This is a paper summarizing the state of research on a topic. These can often be somewhat long, are often but not always by invitation only, and this category can include meta-analysis, but doesn't have to. This may also be the umbrella that commentaries fall under, but again, not always.)

• Letter to the Editor: Letters to the editor will be considered if they give significant comments on recent articles published in Iranian Journal of Biotechnology (IJB) or they contain relevant preliminary data providing new insights. Letters need no abstract and must cite the previous article in IJB that is being discussed, and should have no more than 10 references.



Language Editing

 Please write your text in good English. In case of requirement for language editing (LE), that will be decided and informed by the IJB executive committee, the author is expected to send the manuscript to a certified agency for language editing and submit the certificate to IJB in a due date.


 Publication Fee

 ♦ Publication fee for International Authors

From July 25, 2020 Non-Iranian authors should pay 300 € for publication of their accepted manuscripts. In case of requesting for fast reviewing and publication another 200 € should be paid.

A copy of the payment receipt should be sent to IJB email address( and  email address(  then uploaded on IJB website (Please mention your manuscript ID on the receipt).


Publication fee for Iranian ŮŽAuthors

From July 25, 2020 IJB charges (7,000,000 Rials) Iranian (National) corresponds for publication of their manuscripts. First payment (2,000,000 Rials) with first submission of the manuscript, the second payment (5,000,000 Rials) after manuscript reviewed by reviewers. In case of requesting for fast reviewing and publication another 2,000,000 Rials should be paid.

Payments could be done through Bank account number of Central Bank of the Islamic Republic of Iran with following information:

Account No: 4001024103007689

 Deposit ID: 307024182114504000000000000022

Sheba code: IR680100004001024103007689 

A copy of the payment receipt should be sent to IJB email address and uploaded on IJB website (Please mention your manuscript ID on the receipt).





Iranian Journal of Biotechnology applies the Creative Commons Attribution License (CC 4.0) to works we publish. Under this license, MISRC and MMESA retain ownership of the copyright for their content, but permits unrestricted commercial use, distribution, and reproduction of the article in any medium, provided that the original work is properly cited. No permission is required from the authors or the publisher. For any reuse or redistribution of a work, users must also make clear the license terms under which the work was published. This broad license was developed to facilitate free access to, and unrestricted reuse of, original works of all types. Applying this standard license to your own work will ensure that it is freely and openly available in perpetuity.