@article { author = {Hajinia, Elmira and Fatemi, Seyed Safa-ali and Karkhane, Ali Asghar and Safekordi, Ali Akbar and Yakhchali, Bagher}, title = {Optimization of secretory expression of recombinant hGM-CSF in high cell density cultivation of recombinant Escherichia coli using Taguchi statistical method}, journal = {Iranian Journal of Biotechnology}, volume = {10}, number = {4}, pages = {275-280}, year = {2012}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {Human granulocyte macrophage colony stimulating factor (hGM-CSF) has many therapeutic applications.In this study, in order to verify the purification process, the effect of carbon source, IPTG concentration andpost-induction time on the secretion of recombinant hGM-CSF into the culture medium by recombinantEscherichia coli during high cell density cultivation were evaluated by using the Taguchi statistical method. The results indicated that glucose, 1mM IPTG and a time of 6 h post-induction, represented optimum conditions. The secreted hGM-CSF, overall volumetric productivity and purified hGM-CSF were 373 mg/l, 18 mg/l/h and 63 mg/l, respectively.}, keywords = {Escherichia coli,Fed-batch,High cell density cultivation,Human granulocyte macrophage colony stimulating factor,optimization,Purification}, url = {https://www.ijbiotech.com/article_7190.html}, eprint = {https://www.ijbiotech.com/article_7190_57c232efb7cc443241a465c5edbddc02.pdf} }