Production and first assessment of Iranain secondary tritipyrum genotypes (ISTG) by GISH and AFLP markers in Iran and Germany

Document Type: Research Paper

Authors

1 Department of Crop Production and Plant Breeding, School of Agriculture, Shiraz University, Shiraz 7144165186 Iran

2 Department of genebank, leibniz institute of plant genetics and crop plant research (IPK), corrensstraβe3, D-06466 gatersleben, Germany

3 Department of Cytogenetics and Genome analysis, leibniz institute of plant genetics and crop plant research (IPK), corrensstraβe 3, D-06466 gatersleben, Germany

4 Department of genebank, leibniz institute of plant genetics and crop plant research (IPK), corrensstraβe 3, D-06466 gatersleben, Germany

Abstract

Production and first assessment of Iranian secondary tritipyrum genotypes (ISTG) by GISH and AFLP markers in Iran and Germany

Hossein Shahsavand Hassani1&2, Frank R. Blattner3, Andreas Houben3 and Andreas Bӧrner3
1Department of Agronomy and Plant breeding, College of Agriculture, Shahid Bahonar University of Kerman (SBUK), Kerman, Iran.
2Department of Crop Production and Plant Breeding, College of Agriculture, Shiraz University, Shiraz, Iran
3Genebank Department, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Correns str. 3, D-06466, Gatersleben, Germany.
Corresponding author: Hossein Shahsavand Hassani, Department of Crop Production and Plant Breeding, College of Agriculture, Shiraz University, Shiraz, Iran, Fax No: 07132286134, Cell No: 09131415448, Shahsavand@shirazu.ac.ir

Background: Non-Iranian Primary Tritipyrum (2n=6x=42, AABBEbEb) set seed after Triticale (2n=6x=42, AABBRR) and Tritordeum (2n=6x=42, AABBHcHc) but, due to a few undesirable agronomic traits, it cannot fulfil the commercial expectations of farming.
Objectives: To remove these deficiencies, six hexaploid Tritipyrum lines were crossed with four Iranian bread wheat cultivars which led to the production of 107 (F1), 479 (F2), 768 (F3), and 1539 (F4) Iranian Secondary Tritipyrum Genotypes (ISTG) seeds. This study was carried out for selecting the plants potentially carry the 5Eb chromosome/s and are good candidates for salt tolerant by GISH and RFLP markers.
Material and Methods: The procedure involved extracting the total DNA content of 209 plants, including non-Iranian primary Tritipyrum lines, Iranian wheat cultivars, Chinese Spring addition, and substitution lines for 5Eb and Iranian secondary Tritipyrum genotypes (ISTG: F1, F2, F3, F4). Genomic in situ Hybridization (GISH) on mitotic spreads of fertile new Iranian secondary Tritipyrum genotypes (ISTG) was carried out to demonstrate the feasibility of single Eb chromosomes. There were three trials of 18 Fragment Length Polymorphism (AFLP) EcoRI/MseI primers to identify the presence of the 5Eb chromosome in 105 ISTG plants, along with four wheat addition lines and substitution lines for the 5Eb chromosome.
Results: GISH on mitotic spreads demonstrated the feasibility of producing 75 plants out of 105 fertile new Iranian secondary Tritipyrum genotypes (ISTG) with 0-14 single Eb chromosomes. Among the mentioned markers, only the E36/M59 marker showed 43, 50, 30 and 47 identical bands, respectively, in contrast to 53 expected bands in all plants with the 5Eb chromosome which indicated 21, 33, 9 and 6 out of 75 ISTG plants, respectively, with the 5Eb chromosome.
Conclusions: This study indicated that 69 ISTG Tritipyrum plants were potentially carry the 5Eb chromosome/s and are good candidates for salt tolerant tests in comparison with Iranian modern bread wheat cultivars.
Keywords: AFLP- GISH- ISTG- Non-Iranian Primary Tritipyrum- Thinopyrum bessarabicum- Wheat.

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