Probiotic Bacteria in Wound Healing; An in-vivo study

Document Type: Research Paper


1 Senior research fellow VIT UNIVERSITY

2 Biomolecules lab, VIT University

3 School of Biosciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India


Background: Probiotics are food supplements, which benefits the host by improving its intestinal microbial balance. Probiotics are used as diet supplements to prevent diarrhoea and improve lactose tolerance.
Objective: The present study deals with isolation of a potent probiotic strain capable of inducing healing properties in rat model.
Method: Probiotic VITSAMJ1 was isolated from goat milk using MRS media. The antimicrobial assay was carried out against S. aureus (MTCC 3160) and the wound healing properties were assessed on female Wistar rats. A 1.5 cm2 subcutaneous wound was induced upon the rats, and probiotic gel formulation was tropically applied onto the wounds and tissues biopsy was carried out after 1, 3, 5, 7, 9 and 11th days. Total leucocyte count and Histopathological analysis were performed after each interval.
Results: VITSAMJ1 displayed a zone of inhibition of 22 mm. The groups treated with probiotic gel showed faster healing as compared to the control set. Histopathological examination suggested that the groups treated with probiotic gel formulation showed the significantly higher level of total leucocyte count and an improved wound healing process. By 3rd day the leucocyte counts of the wounded rat treated with the effective isolates VITSAMJ1 was much higher (11,000 uL-1) as compared to the controls (7,000 uL-1).
Conclusion: VITSAMJ1 can be effectively used as a probiotic ointment for wound healing.


Main Subjects