Document Type : Research Paper
Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Department of Chemistry, Faculty of Sciences, Kerman Branch, Islamic Azad University, Kerman, Iran.
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
Background: Magnetic nanoparticles (MNPs) loaded by various active compounds can be used for targeted drug delivery.
Objectives: In the present study, the Fe3O4 magnetic nanoparticles that contained gentamicin were prepared and their antibacterial activities were studied.
Materials and Methods: MNPs containing gentamicin (G@SA-MNPs) were prepared using sodium alginate (SA) as a surface modifier. After and before coating, the prepared MNPs were characterized using transmission electron microscopy (TEM), X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), and vibrating sample magnetometer (VSM). Finally, the antibacterial effect of the MNPs was investigated by a conventional serial agar dilution method.
Results: Particle size distribution analysis showed that the size of MNPs, before and after coating, was in the range of 1-18 nm and 12-40 nm, respectively. The magnetization curve of G@SA-MNPs (with saturation magnetization of 27.9 emu.g-1) confirmed ferromagnetic property. Loading gentamicin on the surface of MNPs was qualitatively verified by FTIR spectrum. Quantitative analysis measurements indicated the gentamicin loading on SA-MNPs as 56.7 ± 5.4%. The measured MICs of G@SA-MNPs for Pseudomonas aeruginosa (PTTC 1574) was 1.28 μg.mL-1. The sub-MIC (0.64 μg.mL-1) concentration of G@SA-MNPs in nutrient broth could successfully inhibit the growth of P. aeruginosa for 14 hours.
Conclusions: Loading gentamicin on the SA-MNPs exhibited reasonable antibacterial effects against P. aeruginosa.