Characterization of the full length coat protein gene of Iranian Grapevine fanleaf virus isolates, genetic variation and phylogenetic analysis

Document Type: Research Paper

Authors

1 Plant Protection Department, University of Tabriz, P.O. Box 345, Tabriz, I.R. Iran.

2 Agricultural Research Center, West Azarbaijan, P.O. Box 365, Uromieh, I.R. Iran.

Abstract

The full-length coat protein gene of Grapevine fanleaf virus (GFLV) isolates from Iran was characterized by
reverse transcription polymerase chain reaction (RTPCR) and sequencing. The expected 1515 bp coat
protein (CP) gene amplicon was obtained for 16 isolates out of 89 that were identified by double antibody
sandwich enzyme-linked immunesorbent assay (DASELISA) in a population of 330 symptomatic grapevine
leaf samples. CP products of eight isolates were cloned and nucleotide sequences were determined.
Parsimonious trees indicated that GFLV isolates from Iran formed a distinct cluster, suggesting an independent evolution.

Keywords