Sokhandan Bashir, N., Pashaei, A., Doulati-Baneh, H. (2011). Characterization of the full length coat protein gene of Iranian Grapevine fanleaf virus isolates, genetic variation and phylogenetic analysis. Iranian Journal of Biotechnology, 9(3), 213-221.
Nemat Sokhandan Bashir; Alireza Pashaei; Hamed Doulati-Baneh. "Characterization of the full length coat protein gene of Iranian Grapevine fanleaf virus isolates, genetic variation and phylogenetic analysis". Iranian Journal of Biotechnology, 9, 3, 2011, 213-221.
Sokhandan Bashir, N., Pashaei, A., Doulati-Baneh, H. (2011). 'Characterization of the full length coat protein gene of Iranian Grapevine fanleaf virus isolates, genetic variation and phylogenetic analysis', Iranian Journal of Biotechnology, 9(3), pp. 213-221.
Sokhandan Bashir, N., Pashaei, A., Doulati-Baneh, H. Characterization of the full length coat protein gene of Iranian Grapevine fanleaf virus isolates, genetic variation and phylogenetic analysis. Iranian Journal of Biotechnology, 2011; 9(3): 213-221.
Characterization of the full length coat protein gene of Iranian Grapevine fanleaf virus isolates, genetic variation and phylogenetic analysis
1Plant Protection Department, University of Tabriz, P.O. Box 345, Tabriz, I.R. Iran.
2Agricultural Research Center, West Azarbaijan, P.O. Box 365, Uromieh, I.R. Iran.
Abstract
The full-length coat protein gene of Grapevine fanleaf virus (GFLV) isolates from Iran was characterized by reverse transcription polymerase chain reaction (RTPCR) and sequencing. The expected 1515 bp coat protein (CP) gene amplicon was obtained for 16 isolates out of 89 that were identified by double antibody sandwich enzyme-linked immunesorbent assay (DASELISA) in a population of 330 symptomatic grapevine leaf samples. CP products of eight isolates were cloned and nucleotide sequences were determined. Parsimonious trees indicated that GFLV isolates from Iran formed a distinct cluster, suggesting an independent evolution.
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