Polymorphism of Prion Protein Gene (PRNP) in Iranian Holstein and two local cattle populations (Golpayegani and Sistani) of Iran

Document Type: Research Paper


1 Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, P.O. Box 4111, I.R. Iran.

2 Department of Biotechnology, Animal Science Research Institute of Iran, Karaj, P.O. Box 1483, I.R. Iran.


Bovine spongiform encephalopathy (BSE) is a fatal infectious neurodegenerative disease in cattle, characterized by the accumulation of an abnormal, proteaseresistant prion protein (PrPSc) in the brain. BSE is similar to scrapie in sheep and goats and Creuzfeldt-Jakob disease in humans. Susceptibility in cattle has
been shown to be under the influence of two polymorphic locations, which are a 23 bp in/del polymorphism
and a 12 bp indel within intron 1 of the prion protein gene (PRNP). DNA was extracted from blood samples
of three Iranian cattle populations including Sistani (Bos indicus) (n=60), Golpayegani (Bos indicus) (n=62) and Iranian Holstein (Bos taurus) (n=50), In order to identify the putative polymorphisms of the PRNP gene of those breeds. Allele, genotype and haplotype frequencies of the polymorphisms were determined for the three populations. Susceptibility analysis was considered as per literature, upon which, it was suggested that the two Bos indicus native populations are more resistant to BSE than the Iranian Holstein (Bos taurus), due to higher gene frequency for insertion allele of the intron 1 of the PRNP gene polymorphism.