Callus induction and shoot regeneration from epicotyl explants of ethnomedicinally important Caesalpinia bonduc (L.) Roxb

Document Type: Research Paper

Authors

1 Post graduate and Research Department of Botany, St. Thomas College, Pala, Arunapuram (P.O), PIN- 686 574, Kottayam (Dt.), Kerala, India.

2 The Rapinat Herbarium and Centre for Molecular Systematics and Taxonomy, St. Joseph’s College (Autonomous). Tiruchirappalli-620 002, Tamil Nadu, India.

3 Post graduate and Research Department of Botany, St. Thomas College, Pala, Arunapuram (P.O), PIN- 686 574, Kottayam (Dt.), Kerala, India

4 Field Science Centre for Northern Biosphere, Hokkaido University, Kita 11, Nishi 10, Kita-ku, Sapporo 060-0811, Japan.

Abstract

An efficient plant regeneration method was established for Caesalpinia bonduc (L.) Roxb. by culturing
immature epicotyl explants. Morphogenic calli were initiated from 96% of epicotyl explants on MS medium
supplemented with BA (6-benzylaminopurine) 4.0 mg/l and NAA (Naphthalene acetic acid) 1.0 mg/l. The calli
formed were excised and subcultured on MS medium fortified with 1 mg/l 2, 4-dichlorophenoxyacetic acid (2,
4-D) for further proliferation. Maximum percent organogenesis (84%) and average shoots per culture
(5.6) was observed on MS medium fortified with 3.0 mg/l BA and 1.0 mg/l IAA (indole-3- acetic acid).
Addition of indole-3-butyric acid (IBA) in ½ MS medium favored rooting of recovered shoots. Out of 30 rooted
shoots transferred to soil 27 survived after acclimatization.

Keywords