Molecular Typing of Avian Pasteurella multocida lsolatesby PCR-RFLPof ompH Gene

Document Type: Research Paper

Authors

1 Department of Aerobic Veterinary Bacterial Vaccines, Razi Vaccine and Serum Research Institute, Hesarak, Karaj, I.R. Iran

2 Department of Biotechnology, Razi Vaccine and Serum Research Institute, Hesarak, Karaj, I.R. Iran.

Abstract

Molecular typing of twenty-five Pasteurella multocida isolates has been assessed by restriction fragment
length polymorphism (RFLP) of a species-specific PCR assay. Amplification was based on the gene ompH, encoding a major outer memberane protein. RFLP analysis of the 1.2 kb ompH-amplification using
EcoRI, HindIII and CfoI endonucleases produced 7 different patterns for the twenty five isolates of the four
P.multocida serotypes. The PCR-RFLP of the ompH gene was found to be potentially a useful method for
typing of P. multocida and therefore, for studying the epidemiology of P. multocida infections.

Keywords