Document Type: Research Paper
Department of Aerobic Veterinary Bacterial Vaccines, Razi Vaccine and Serum Research Institute, Hesarak, Karaj, I.R. Iran
Department of Biotechnology, Razi Vaccine and Serum Research Institute, Hesarak, Karaj, I.R. Iran.
Molecular typing of twenty-five Pasteurella multocida isolates has been assessed by restriction fragment
length polymorphism (RFLP) of a species-specific PCR assay. Amplification was based on the gene ompH, encoding a major outer memberane protein. RFLP analysis of the 1.2 kb ompH-amplification using
EcoRI, HindIII and CfoI endonucleases produced 7 different patterns for the twenty five isolates of the four
P.multocida serotypes. The PCR-RFLP of the ompH gene was found to be potentially a useful method for
typing of P. multocida and therefore, for studying the epidemiology of P. multocida infections.