Document Type: Brief Report
Animal Science Department, College of Agriculture, Ferdowsi University of Mashhad, P.O. Box: 91775-1163 Mashhad, I.R. Iran.
Center of Animal Breeding in North-Eastern of Iran, Jahad-Keshavarzi Khorasan, Mashhad, I.R. Iran
To prevent distribution of recessive alleles in dairy herds all bulls used for AI (Artificial Insemination) have
to be tested. In this study 26 blood and 4 semen samples were supplied from Iranian Holstein bulls used for
AI. Genomic DNA was extracted from 100 μl of blood and 200 μl of semen. Samples were tested by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. PCR reaction was performed for amplification of polymorphic region of the CD18 gene on chromosome 1 and exon 5 of ASS gene on chromosome 11. We detected one Bovine Leukocyte Adhesion Deficiency (BLAD) carrier and no carrier for bovine Citrolliemia in this study. Hardy-Weinberg test confirmed the equilibrium of BLAD locus in this population.