Survey of Efficiency of Six Microsatellite Loci in Iranian Indigenous Cattle and Buffalo Populations

Document Type: Research Paper

Authors

1 Department of Animal Sciences, Guilan University, P.O.Box: 1841, Rasht, I.R. Iran.

2 Agricultural Biotechnology Research Institute of Iran, P.O.Box: 31535-1892, Karaj, I.R. Iran.

3 Agricultural Biotechnology Research Institute of Iran, P.O.Box: 31535-1892, Karaj, I.R. Iran

Abstract

Genetic diversity of three native buffalo populations from Azarie, Mazandaranie and Khuzestanie and two
Iranian cattle breeds namely Sistanie and Taleshie were estimated using six microsatellite markers. Thirty
individuals were randomly selected from each population/ breed. Total genomic DNA was extracted by an
optimized phenol–chloroform extraction method. The extracted DNA was amplified through polymerase
chain reaction (PCR). Of the six microsatellite loci used in this study, two loci (ETH10 and ETH 225) were
monomorphic within the three buffalo populations. Genetic distance between the populations was estimated
by Fst (two by two) method. Maximum genetic distance was observed between Khuzestanie and
Mazandaranie buffalo populations (55%); whereas the minimum genetic distance (31%) was observed
between Khuzestanie and Azarie populations. Values of both polymorphic information content (PIC) and heterozygosity (observed and expected) were higher within two cattle breeds as compared to those estimated
for three buffalo populations.

Keywords