Cloning and Sequencing of Desulfurization Operon froma Newly Isolated Bacterium Rhodococcus FMF

Document Type: Research Paper

Authors

National Research Center for Genetic Engineering and Biotechnology, P.O. Box: 14155-6343, Tehran, I.R. Iran.

Abstract

A native strain of Rhodococcus FMF was isolated from soil samples collected from Tabriz petroleum refinery
area in Iran. The presence of sox operon in the genomic DNA and the ability of bacteria to consume
dibenzothiophen (DBT) as sulfur source were assessed. DNA was amplified by PCR and subsequently cloned into pTZ57R cloning vector. Diffrent restriction endonucleases; EcoRI, HindIII, EcoRI/HindIII and XhoI were used to prove the accuracy of cloning. Acquired clone was named pTZAB57R. Subsequently, the relevant A and B genes involved in DBT consumption were sequenced and compared with the map of Rhodococcus erythropolis IGTS8 desulfurization pathway genes. Results show that the desulfurization operon in the native isolated Rhodococcus FMF bacterium is completely conserved.

Keywords