A Novel Multi-Epitope Edible Vaccine Candidate for Newcastle Disease Virus: In Silico Approach

Document Type : Research Paper

Authors

1 Department of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.

2 Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.

3 Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.

Abstract

Background: Newcastle disease, is one of the most important illnesses in the aviculture industry which shows a constant 
threat. In this case, the vaccine could be considered an important solution to prevent and control this disease. So, the 
development of a new and more effective vaccine against Newcastle disease is an urgent need. Immune informatics is an 
important field that provides insight into the experimental procedure and could facilitate the analysis of large amounts of immunological data generated by experimental research and help to design a new vaccine candidate. 
Objectives: This study is aimed at bioinformatics to investigate and select the most immunogenic and conserved epitopes derived from F and HN glycoproteins, which play a key role in pathogenesis and immunity. This strategy could cover a 
wide range of Newcastle disease viruses.
Materials and Method: For expression in both E. coli (as an injectable recombinant vaccine candidate) and maize plant 
(as an edible vaccine candidate) host, two constructs were designed and analyzed separately. Furthermore, the role of 
LTB as an effective bio-adjuvant for general eliciting of the immune system and simultaneous expressions with those 
two antigens was evaluated. Hence, here a multimeric recombinant protein with the abbreviation LHN2F from the highly 
immunogenic part of HN, F and LTB proteins were designed. The synthetic construct was analyzed based on different 
bioinformatics tools. 
Results: The proper immunogenicity and stability of this multimeric fusion protein have been shown by immunoinformatic methods from various servers. To confirm the function of the designed protein, the final molecule was docked to chicken MHC class I using the Pyrex-python 0.8 program. the results of Immune Epitope analysis were confirmed by the docking results between protein and receptor.
Conclusions: ‎The results of structural and immunological computational studies proposed that the protein deduced from 
this novel construct could act as a vaccine candidate for Newcastle disease virus‎ control and prophylactic.

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