Construction of a nanobodies phage display library from an Escherichia coli immunized dromedary

Document Type: Research Paper

Authors

1 Arid lands institute

2 Livestock and wildlife laboratory, Arid lands Institute, 4119 Medenine, Tunisia

10.30498/ijb.2020.127753.2247

Abstract

Background: Diarrhea caused by Escherichia coli is a major cause of morbidity and mortality in young animals. Few treatment options are available, mainly antibiotic therapy increasingly limited by resistance to commonly used drugs.
Objectives: The aim of this work was to develop an immunotherapy based on the use of camel VHH antibody fragments, or nanobodies, to target pathogenic E. coli surface antigens.
Material and methods: We immunized a camel with a killed strain we had previously isolated from a diarrheic camel calf and identified as expressing the F17 fimbriae antigen.
Results: The immunized animal developed an anti-E. coli immune response including heavy chain antibodies. Lymphocytes from this animal were purified and RNA isolated to create a VHH library by phage display with a size of about 109 individual transformants. Panning on live E. coli cells resulted in the isolation of VHH fragments specific to the cell surface antigens.
Conclusion: The identification of these antigens can lead to the development of new diagnostic and therapeutic tools against diarrhea.

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