Comparative Proteomic Analysis of Two Manilkara Species Leaves Under NaCl Stress

Document Type : Research Paper


1 Xiamen overseas Chinese subtropical plant introduction garden, Xiamen, Fujian, China

2 Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, Xiamen University, Xiamen, Fujian, China

3 Fujian Institute of Subtropical Botany, Xiamen, Fujian, China


Background: Salinity is a major environmental limiting factor, which affect agricultural production. The two Manilkara seedlings (M. roxburghiana and M. zapota) with high economic importance, could not adapt well to higher soil salinity and little is known about their proteomic mechanisms.
Objectives: The mechanisms responsible for the effects of salinity on the two Manilkara species leaves were examined by means of proteomic analysis.
Material and Methods: The seedlings were cultivated in a greenhouse and treated with NaCl. Leaves of control and the salt-stressed seedlings were sampled for phenol protein extraction. Proteins were separated by two-dimensional gel electrophoresis coupled with mass spectroscopy to study the change of proteins under different NaCl concentration.
Results: For M. roxburghiana leaves, 21 protein spots exhibited significant abundance variations between the control and the 6‰, 8‰ NaCl treatments, of these 13 proteins were identified. They included L-ascorbate peroxidase, chloroplast carbonic anhydrase, phosphoglycerate kinase, 5 heat-shock proteins(HSPs) which were all down-regulated; For M. zapota leaves, 35 protein spots exhibited significant abundance variations, then 24 proteins were identified, including 7 down-regulated HSPs as well as glyceraldehyde-3-phosphate dehydrogenase, Cell division protein, putative mitochondrial NAD-dependent malate dehydrogenase, ATP synthase, Rubisco large subunit-binding protein, Cytochrome c peroxidase.
Conclusions: Based on the common identified proteins between the two M. species, our results indicated that the identificated proteins in the two Manilkara species were involved in carbohydrate metabolism, photosynthesis, defense and stress. HSPs exhibited variation strictly related to NaCl stress. The down-regulated HSPs meant the function to repair cells that have suffered damage weaken during stress process. Furthermore, except for HSP70 in M. zapota leaves, the HSPs in the two species were all small heat shock proteins (sHSPs) with molecular weights ranging from 15 to 42 kDa.


Main Subjects

1.           Bafana A, Devi SS, Chakrabarti T. Azo dyes: past, present and the future. Environ Rev. 2011;19(NA):350-371. doi: 10.1139/a11-018
2.           Zollinger H. Color chemistry: syntheses, properties, and applications of organic dyes and pigments. New York: VCH; 1987.
3.           Chavan R. Indian textile industry. Indian J Fibre Textile Res. 2001;26:11-21.
4.           Bafana A, Chakrabarti T, Devi SS. Azoreductase and dye detoxification activities of Bacillus velezensis strain AB. Appl Microbiol Biotechnol. 2008;77(5):1139-1144. doi: 10.1007/s00253-007-1212-5 pmid: 18034237
5.           Shertate RS, Thorat P. Biotransformation of Textile Dyes: A Bioremedial Aspect of Marine Environment. Am J Environ Sci. 2014;10(5):489-499. doi: 10.3844/ajessp.2014.489.499
6.           Seshadri S, Bishop PL, Agha AM. Anaerobic/aerobic treatment of selected azo dyes in wastewater. Waste Manage. 1994;14(2):127-137. doi: 10.1016/0956-053x(94)90005-1
7.           Shah K. Biodegradation of Azo dye compounds. Int Res J Biochem Biotechnol. 2014;1(2):5-13.
8.           Senan RC, Abraham TE. Bioremediation of textile azo dyes by aerobic bacterial consortium. Biodegradation. 2004;15(4):275-280. pmid: 15473556
9.           Bagewadi ZK, Vernekar AG, Patil AY, Limaye AA, Jain VM. Biodegradation of industrially important textile dyes by actinomycetes isolated from activated sludge. Biotechnol Bioinf Bioeng. 2011;1(3):351-360.
10.        Subramani R, Aalbersberg W. Marine actinomycetes: an ongoing source of novel bioactive metabolites. Microbiol Res. 2012;167(10):571-580. doi: 10.1016/j.micres.2012.06.005 pmid: 22796410
11.        McCarthy AJ, Williams ST. Actinomycetes as agents of biodegradation in the environment — a review. Gene. 1992;115(1-2):189-192. doi: 10.1016/0378-1119(92)90558-7
12.        Pasti-Grigsby MB, Lewis TA, Crawford DL, Crawford RL. Transformation of 2,4,6-trinitrotoluene (TNT) by actinomycetes isolated from TNT-contaminated and uncontaminated environments. Appl Environ Microbiol. 1996;62(3):1120-1123. pmid: 8975606
13.        Wackett LP, Brusseau GA, Householder SR, Hanson RS. Survey of microbial oxygenases: trichloroethylene degradation by propane-oxidizing bacteria. Appl Environ Microbiol. 1989;55(11):2960-2964. pmid: 2624467
14.        Klausmeier R, Osman J, editors. Biodegradation of plastics by actinomycetes. Proceedings of the third International Biodegradation Symposium; 1976; London: Applied Science Publishers.
15.        Zhou W, Zimmermann W. Decolorization of industrial effluents containing reactive dyes by actinomycetes. FEMS Microbiol Lett. 1993;107(2-3):157-161. doi: 10.1111/j.1574-6968.1993.tb06023.x pmid: 8472899
16.        Karthik L, Kumar G, Bhaskara Rao K. Diversity of marine actinomycetes from Nicobar marine sediments and its antifungal activity. Int J Pharm Pharm Sci. 2010;2(1):199-203.
17.        Priyaragini S, Veena S, Swetha D, Karthik L, Kumar G, Bhaskara Rao KV. Evaluating the effectiveness of marine actinobacterial extract and its mediated titanium dioxide nanoparticles in the degradation of azo dyes. J Environ Sci (China). 2014;26(4):775-782. doi: 10.1016/S1001-0742(13)60470-2 pmid: 25079407
18.        Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol. 1966;16(3):313-340. doi: 10.1099/00207713-16-3-313
19.        Singh S, Chatterji S, Nandini PT, Prasad ASA, Rao KVB. Biodegradation of azo dye Direct Orange 16 by Micrococcus luteus strain SSN2. Int J Environ Sci Technol. 2014;12(7):2161-2168. doi: 10.1007/s13762-014-0588-x
20.        Raja MMM, Raja A, Salique SM, Gajalakshmi P. Studies on effect of marine actinomycetes on amido black (azo dye) decolorization. J Chem Pharmac Res. 2016;8(8):640-644.
21.        Chakravarthy B, Vijayasree J, Swathi V, Sudhira D, Uma Maheswari Devi P. Screening and exploration of azo dye decolorizing actinomycetes from marine sediments. Int J Sci Eng Res. 2015;6(2):27-30.
22.        Shobana S, hangam BT. Biodegradation and Decolorization of Reactive Orange 16 by Nocardiopsis alba Soil Isolate. J Bioremed Biodegrad. 2012;03(06). doi: 10.4172/2155-6199.1000155
23.        Lakshmipathy TD, Prasad AA, Kannabiran K. Production of biosurfactant and heavy metal resistance activity of Streptomyces sp. VITDDK3-a novel halo tolerant actinomycetes isolated from saltpan soil. Biol Res. 2010;4(2):108-115.
24.        Saratale RG, Saratale GD, Chang JS, Govindwar SP. Bacterial decolorization and degradation of azo dyes: A review. J Taiwan Instit Chem Eng. 2011;42(1):138-157. doi: 10.1016/j.jtice.2010.06.006
25.        Mane U, Gurav P, Deshmukh A, Govindwar S. Degradation of textile dye reactive navy–blue Rx (Reactive blue–59) by an isolated Actinomycete Streptomyces krainskii SUK–5. Malaysian J Microbiol. 2008;4(2):1-5.
26.        Lu L, Zeng G, Fan C, Ren X, Wang C, Zhao Q, et al. Characterization of a laccase-like multicopper oxidase from newly isolated Streptomyces sp. C1 in agricultural waste compost and enzymatic decolorization of azo dyes. Biochem Eng J. 2013;72:70-76. doi: 10.1016/j.bej.2013.01.004
27.        Babu SS, Mohandass C, Vijayaraj A, Dhale MA. Detoxification and color removal of Congo Red by a novel Dietzia sp.(DTS26)–a microcosm approach. Ecotoxicol Environ Saf. 2015;114:52-60.
28.        Suzuki T, Endo K, Ito M, Tsujibo H, Miyamoto K, Inamori Y. A thermostable laccase from Streptomyces lavendulae REN-7: purification, characterization, nucleotide sequence, and expression. Biosci Biotechnol Biochem. 2003;67(10):2167-2175. doi: 10.1271/bbb.67.2167 pmid: 14586105
29.        Molina-Guijarro JM, Perez J, Munoz-Dorado J, Guillen F, Moya R, Hernandez M, et al. Detoxification of azo dyes by a novel pH-versatile, salt-resistant laccase from Streptomyces ipomoea. Int Microbiol. 2009;12(1):13-21. pmid: 19440979
30.        Endo K, Hayashi Y, Hibi T, Hosono K, Beppu T, Ueda K. Enzymological characterization of EpoA, a laccase-like phenol oxidase produced by Streptomyces griseus. J Biochem. 2003;133(5):671-677. doi: 10.1093/jb/mvg086 pmid: 12801920
31.        Mostafa M. Waste water treatment in textile Industries-the concept and current removal technologies. J Biodivers Environ Sci. 2015;7(1):501-525.
32.        Sahasrabudhe M, Pathade G. Biodegradation of azo dye CI Reactive Orange 16 by an actinobacterium Georgenia sp. CC-NMPT-T3. Intl J Adv Res 1: 91–99, 2013.
33.        Saratale RG, Saratale GD, Kalyani DC, Chang JS, Govindwar SP. Enhanced decolorization and biodegradation of textile azo dye Scarlet R by using developed microbial consortium-GR. Bioresour Technol. 2009;100(9):2493-2500. doi: 10.1016/j.biortech.2008.12.013 pmid: 19157864
34.        Kalyani DC, Telke AA, Dhanve RS, Jadhav JP. Ecofriendly biodegradation and detoxification of Reactive Red 2 textile dye by newly isolated Pseudomonas sp. SUK1. J Hazard Mater. 2009;163(2-3):735-742. doi: 10.1016/j.jhazmat.2008.07.020 pmid: 18718713
35.        Sahasrabudhe MM, Saratale RG, Saratale GD, Pathade GR. Decolorization and detoxification of sulfonated toxic diazo dye C.I. Direct Red 81 by Enterococcus faecalis YZ 66. J Environ Health Sci Eng. 2014;12(1):151. doi: 10.1186/s40201-014-0151-1 pmid: 25649265
36.        Ilyas S, Rehman A. Decolorization and detoxification of Synozol red HF-6BN azo dye, by Aspergillus niger and Nigrospora sp. Iranian J Environ Health Sci Eng. 2013;10(1):12. doi: 10.1186/1735-2746-10-12 pmid: 23369298
37.        Lade H, Govindwar S, Paul D. Low-Cost Biodegradation and Detoxification of Textile Azo Dye C.I. Reactive Blue 172 byProvidencia rettgeriStrain HSL1. J Chem. 2015;2015:1-10. doi: 10.1155/2015/894109