Optimization of secretory expression of recombinant hGM-CSF in high cell density cultivation of recombinant Escherichia coli using Taguchi statistical method

Authors

1 Department of Chemical Engineering, Science and Research Branch, Islamic Azad University, P.O. Box 1477893855, Tehran, I.R. Iran.

2 Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14965/161, Tehran, I.R. Iran.

Abstract

Human granulocyte macrophage colony stimulating factor (hGM-CSF) has many therapeutic applications.
In this study, in order to verify the purification process, the effect of carbon source, IPTG concentration and
post-induction time on the secretion of recombinant hGM-CSF into the culture medium by recombinant
Escherichia coli during high cell density cultivation were evaluated by using the Taguchi statistical method. The results indicated that glucose, 1mM IPTG and a time of 6 h post-induction, represented optimum conditions. The secreted hGM-CSF, overall volumetric productivity and purified hGM-CSF were 373 mg/l, 18 mg/l/h and 63 mg/l, respectively.

Keywords

Iranian Journal of Biotechnology
Volume 10, Issue 4
October 2012
Pages 275-280

Files

Share

How to cite

Statistics