Genetic variability of calpastatin and calpain genes in Iranian Zel sheep using PCR-RFLP and PCR-SSCP methods

Document Type : Brief Report


1 Department of Animal Science, Gorgan University of Agricultural Sciences and Natural Resources, P.O. Box 4913815739, Gorgan, I.R. Iran.

2 Department of Animal Science, Ferdowsi University of Mashhad, P.O. Box 9177948978, Mashhad, I.R. Iran.

3 Golestan Agriculture Jahad, P.O. Box 49174, Gorgan, I.R. Iran.

4 Department of Animal Science, Faculty of Gonbad, P.O. Box 4971799151, Gonbad, I.R. Iran.


The genotypes for calpastatin (CAST) and calpain (CAPN) loci were determined by PCR-RFLP and PCRSSCP
methods. Blood samples were collected from 200 pure-bred Zel sheep from Shirang’s Zel sheep Breeding Station located in south-west of Golestan, Iran. Extraction of genomic DNA was performed based on the modified salting out method. Based on results, two investigated loci were polymorphic and had different
gene variants. Heterozygosis was low for both loci. Chi-square test confirmed Hardy-Weinberg equilibrium
only in CAST locus using SSCP method. Detected polymorphisms and associations of genetic variation
with meat production and tenderness may help to find the effective genotypes of Zel sheep for the economic