Cloning, expression and purification of Clostridium botulinum neurotoxin type E binding domain


1 Department of Biology, Faculty of Science, Imam Hossain University, Tehran, I.R. Iran.

2 Department of Biology, Faculty of Science, Tehran University, Tehran, I.R. Iran.

3 Department of Biology, College of Basic Science, Shahed University, Tehran, I.R. Iran.


Botulinum neurotoxins constitute a family of bacterial toxins for botulism syndrome in human. The toxins
bind with high affinity to nerve cells where they cause a complete inhibition and release of neurotransmitters
and thereby produce flaccid paralysis. In this study the binding domain of type E neurotoxin was isolated by
PCR and expressed in a proper expression vector. The results of this investigation can be used as a tool
to study the mechanism of binding of holotoxins. This study is also implicated in antibody production against
botulism syndrome.