Biosynthesis of Silver Nanoparticles Using Pine Pollen and Evaluation of the Antifungal Efficiency

Document Type : Research Paper


1 School of Medicine, Bam University of Medical Sciences, Bam, Iran.

2 Research and Development Center, Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3 Institute for Infrastructure Engineering, Western Sydney University, Kingswood Campus, Locked Bag 1797, NSW 2751, Australia

4 Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran

5 Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran



Background: Nanoparticles have been applied to medicine, hygiene, pharmacy and dentistry, and will bring significant advances in the prevention, diagnosis, drug delivery and treatment of disease. Green synthesis of metal nanoparticles has a very important role in nanobiotechnology, allowing production of non-toxic and eco-friendly particles.
Objectives: Green synthesis of silver nanoparticles (AgNPs) was studied using pine pollen as a novel, cost-eff ective, simple and non-hazardous bioresource. The antifungal activity of the synthesized AgNPs was investigated in vitro.
Materials and Methods: Biosynthesis of AgNPs was conducted using pollen of pine (as a novel bioresource) acting as both reducing and capping agents. AgNPs were characterized using UV-visible spectroscopy, X-ray diff raction and transmission electron microscopy. In evaluation for antifungal properties, the synthesized AgNPs represented signifi cant in vitro inhibitory effects on Neofusicoccum parvum cultures.
Results: Pine pollen can mediate biosynthesis of colloidal AgNPs with an average size of 12 nm. AgNPs were formed at 22C and observed to be highly stable up to three months without precipitation or decreased antifungal property. AgNPs showed signifi cant inhibitory eff ects against Neofusicoccum parvum.
Conclusion: The first report for a low-cost, simple, well feasible and eco-friendly procedure for biosynthesis of AgNPs was presented. The synthesized AgNPs by pine pollen were nontoxic and eco-friendly, and can be employed for large-scale production. The nanoparticles showed strong eff ect on quantitative inhibition and disruption of antifungal growth.


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