Detection of Single-Nucleotide Polymorphism in AGPAT6 Gene, Associated with Milk Fat Content, using Tetra-Primer ARMS PCR-Based Assay, in Karan Fries Breeding Bulls

Document Type : Brief Report


1 Krishi Vigyan Kendra, Jhansi, Banda University of Agriculture and Technology, Banda

2 Animal Genetics and Breeding Division, ICAR-National Dairy Research Institute, Karnal- 132001, Haryana, India

3 Animal Biotechnology Division, ICAR-National Dairy Research Institute, Karnal- 132001, Haryana, India

4 Animal Genetics and Breeding Division, Tamil Nadu Veterinary and Animal Sciences University, Chennai- 600051, Tamil Nadu, India


Background: The bovine AGPAT6 gene is one of the potential candidate genes governing milk fat synthesis.
Objectives: Identification of single nucleotide polymorphisms (SNP) in the targeted region of AGPAT6 gene and their effect on expected breeding values (EBV) of first lactation milk production traits viz. fat %, fat yield and 305 days milk yield in Karan Fries (KF) breeding bulls were sought.
Materials and Methods: A tetra-primer ARMS PCR technique was adapted to genotype an SNP, g.36,175,805C>T located on 5’ flanking region of AGPAT6 gene. The relationship between EBV of milk production traits and polymorphic locus of AGPAT6 gene was assessed.
Results: Three kinds of genotype (CC, CT, and TT) with respect to g.36,175,805C>T SNP locus were observed. The identified SNP had significant (P<0.05) influence on EBV of fat % (EBV-FP). The KF bulls with CC and CT genotype had comparatively higher EBV-FP than the bulls with the TT genotype. The substitution of “C” allele by “T” allele led to a decrease of 0.0045 % in the EBV-FP.
Conclusion: The identified SNP was significantly associated with EBV-FP, thus it may be utilized as a molecular marker for developing marker-assisted selection strategy to enhance the milk fat content in KF cattle population.


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