TY - JOUR ID - 7136 TI - Characterization of Iranian isolates of canine parvovirus in fecal samples using polymerase chain reaction assay JO - Iranian Journal of Biotechnology JA - IJB LA - en SN - 1728-3043 AU - Askari Firoozjaii, Hadi AU - Jafari Shoorijeh, Sardar AU - Mohammadi, Ali AU - Tamadon, Amin AD - Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, P.O. Box 71345-1731, Shiraz, I.R. Iran. AD - Department of Pathobiology, School of Veterinary Medicine, Shiraz University, P.O. Box 71345-1731, Shiraz, I.R. Iran. AD - Department of Animal Health Management, School of Veterinary Medicine, Shiraz University, P.O. Box 71345-1731, Shiraz, Iran and Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, I.R. Iran. Y1 - 2011 PY - 2011 VL - 9 IS - 1 SP - 63 EP - 68 KW - Polymerase Chain Reaction KW - Canine Parvovirus KW - Dog KW - Iran DO - N2 - Despite the widespread prevalence of canine parvovirus disease (CPV) in Iranian dog population,molecular diagnosis of CPV variants, and investigation of the trends of its genetic changes is a new effort. Inthis study 50 samples from dogs suspicious of infection with clinical signs of diarrhea and vomiting, and 25samples from dogs suspected of infection with general symptoms such as depression and anorexia werecollected from dogs presented to the veterinary clinic of Shiraz University, Shiraz, Iran. Viral DNA wasextracted from feces. Three specific pairs of primers, P2, Pab, and Pb, were used in a PCR assay for differential diagnosis of the virus type. Pab primer pairs detect the new type-strains, CPV-2a and 2b. Theprimer pairs P2 and Pb detect CPV types 2 and 2b, respectively. Our results showed that 44 individualswith clinical signs of diarrhea and vomiting were positive for CPV-2. 39 individuals (89%) were positive forCPV-2b and 5 individuals (11%) for CPV-2a. Therefore, the CPV-2b was identified as the predominantvirus type. All dogs without symptoms of diarrhea and vomiting were CPV-negative. The relationship ofbreed, age and sex with PCR results was not significant (P>0.05). For the first time in the country, thecausative agent of CPV-2 was identified, and presence of new antigenic variants, CPV-2a and CPV-2b wasconfirmed. UR - https://www.ijbiotech.com/article_7136.html L1 - https://www.ijbiotech.com/article_7136_34e68f932e2917571bd1349129e0e54a.pdf ER -