TY - JOUR ID - 7128 TI - Overexpression of full-length core protein of hepatitis C virus by Escherichia coli cultivated in stirred tank fermentor JO - Iranian Journal of Biotechnology JA - IJB LA - en SN - 1728-3043 AU - Hemmat, Jafar AU - Yakhchali, Bagher AU - Khajeh, Khosro AU - Moosavi-Movahedi, Ali Akbar AU - Karkhane, Ali Asghar AD - National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14965/161, Tehran, I.R. Iran. AD - National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14965/161, Tehran, I.R. Iran. AD - Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, P.O. Box 14115-154, Tehran, I.R. Iran. AD - Institute of Biochemistry and Biophysics, University of Tehran, P.O. Box 13145-1384, Tehran, I.R. Iran Y1 - 2011 PY - 2011 VL - 9 IS - 4 SP - 245 EP - 252 KW - Hepatitis C Virus KW - core protein KW - Overexpression KW - Recombinant protein DO - N2 - The mature core protein of the Hepatitis C virus (HCVC173) carrying pelB as a signal peptide (PelB::core) was overexpressed in Escherichia coli as 18% and 23.3% of the host’s total protein, in flask and fermentor cultivation, respectively. A final specific yield of 25 ± 1 mg HCVC173/g dry cell weight and an overallproductivity of 51±1 mg HCVC173/l/h were obtained in the stirred-tank fermentor. The recombinantPelB::core protein was overexpressed as the inclusion body (IB) form, higher than the expected level whencompared to the HCVC173, which was also showed by the analysis of secondary structure of mRNAs andcalculation of the Codon Adaptation Index of the gene. The results showed that the combined effects of protein fusion and the signal sequence significantly enhanced the production of recombinant matureHCVC173 in E. coli. Therefore, the fusion form of the mature HCV core protein and the conditions defined inthis study provide an alternative strategy for HCVC173 production in high cell density culture of E. coli. UR - https://www.ijbiotech.com/article_7128.html L1 - https://www.ijbiotech.com/article_7128_45c6632f59efadc1fa8df487b69e343e.pdf ER -