TY - JOUR ID - 6942 TI - Survey of Efficiency of Six Microsatellite Loci in Iranian Indigenous Cattle and Buffalo Populations JO - Iranian Journal of Biotechnology JA - IJB LA - en SN - 1728-3043 AU - Mirhoseinie, Seyed-Ziyaeddin AU - Vahidie, Seyed-Mohammad Farhad AU - Gharehyazie, Behzad AD - Department of Animal Sciences, Guilan University, P.O.Box: 1841, Rasht, I.R. Iran. AD - Department of Animal Sciences, Guilan University, P.O.Box: 1841, Rasht, I.R. Iran. AD - Agricultural Biotechnology Research Institute of Iran, P.O.Box: 31535-1892, Karaj, I.R. Iran Y1 - 2005 PY - 2005 VL - 3 IS - 1 SP - 41 EP - 47 KW - genetic diversity KW - Polymorphism KW - Microsatellite markers KW - buffalo KW - Cattle DO - N2 - Genetic diversity of three native buffalo populations from Azarie, Mazandaranie and Khuzestanie and twoIranian cattle breeds namely Sistanie and Taleshie were estimated using six microsatellite markers. Thirtyindividuals were randomly selected from each population/ breed. Total genomic DNA was extracted by anoptimized phenol–chloroform extraction method. The extracted DNA was amplified through polymerasechain reaction (PCR). Of the six microsatellite loci used in this study, two loci (ETH10 and ETH 225) weremonomorphic within the three buffalo populations. Genetic distance between the populations was estimatedby Fst (two by two) method. Maximum genetic distance was observed between Khuzestanie andMazandaranie buffalo populations (55%); whereas the minimum genetic distance (31%) was observedbetween Khuzestanie and Azarie populations. Values of both polymorphic information content (PIC) and heterozygosity (observed and expected) were higher within two cattle breeds as compared to those estimatedfor three buffalo populations. UR - https://www.ijbiotech.com/article_6942.html L1 - https://www.ijbiotech.com/article_6942_5ebb5cac5cc010e1629e4c176e990a08.pdf ER -