TY - JOUR ID - 51471 TI - In silico fusion of epsilon and beta toxin genes of Clostridium perfringens types D and B JO - Iranian Journal of Biotechnology JA - IJB LA - en SN - 1728-3043 AU - Pilehchian Langroudi, Reza AU - Aghaei pour, Khosrow AU - Shamsara, Mahdi AU - Ghorashi, Saied Ali AD - 1Department of Molecular Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Karaj Highway, P.O. Box 14965/161, I.R. Iran. AD - Department of Genomix and Genetic Engineering, Razi Vaccine and Serum Research Institute, Karaj, I.R. Iran. AD - Department of Molecular Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Karaj Highway, P.O. Box 14965/161, I.R. Iran. Y1 - 2012 PY - 2012 VL - 10 IS - 1 SP - 55 EP - 61 KW - Clostridium perfringens KW - epsilon toxin KW - Beta toxin KW - Fusion KW - In Silico DO - N2 - Fusion protein technology represents the strategy to achieve rapid, efficient, and cost-effective proteinexpression. Epsilon and Beta toxins are the most potent Clostridial toxins and cause disease in animals.This study describes in silico fusion of Clostridium perfringens types D and B epsilon and beta toxin genesthat was used for cloning in E.coli. The etx and cpb genes were retrieved from the GenBank and a fusiongene was designed to produce a chimeric fusion protein. Secondary and tertiary structures and specificitiesof fusion protein were determined by online software. Results showed that the designed fusion gene construction is suitable for chimeric fusion protein expression. UR - https://www.ijbiotech.com/article_51471.html L1 - https://www.ijbiotech.com/article_51471_db3f75654d8fa3ab5ae1b718b3cde540.pdf ER -