TY - JOUR ID - 167566 TI - Inhibition of NO Production in LPS-Stimulated Primary Rat Glial Cells by Gnidilatimonoein and Extract of Daphne mucronata JO - Iranian Journal of Biotechnology JA - IJB LA - en SN - 1728-3043 AU - Bahrami salehloo, elham AU - Sabouni, Farzane AU - Mianabadi, Manijheh AD - Departement of Biology, Faculty of Sciences, Golestan University, Gorgan, Iran AD - National Institute of Genetic Engineering and Biotechnology, Tehran, Iran Y1 - 2023 PY - 2023 VL - 21 IS - 2 SP - 52 EP - 62 KW - anti-neuroinflammation KW - Daphne mucronata KW - Glial KW - Gnidilatimonoein KW - nitric oxide DO - 10.30498/ijb.2023.285965.3052 N2 - Background: In the CNS, glial cells are involved in neuroinflammation and neuropathic pain. The glial cells are activated by a variety of pathological conditions and release pro-inflammatory mediators, including nitric oxide (NO). Overexpression of iNOS (inducible nitric oxide synthase) and extra NO is detrimental to neurophysiology and neuronal viability.Objectives: This study aimed to examine the effect of Gnidilatimonein isolated from D. mucronata and its leaves extract (as natural phytochemicals) on NO production in the LPS-induced primary glial cells.Materials and Methods: A preparative HPLC method was used to isolate gnidilatimonoein from leaves ethanolic extract. Various doses of Gnidilatimonoein, the ethanolic extract were applied to primary glial cells inflamed by lipopolysaccharide. A Colorimetric test, an MTT assay, and a RT-PCR analysis were then performed to analyze and compare NO production, cell viability, and iNOS expression.Results: Gnidilatimonoein treatment of pretreated primary glial cells significantly inhibited iNOS expression and decreased NO synthesis. Plant extracts also reduced NO production in inflamed microglial and glial at 0.1-3 mg.mL-1. At these concentrations, none of these compounds exerted a cytotoxic effect, suggesting that their anti-inflammatory effects were not due to the death of cells.Conclusion: This study indicates that D. mucronata and its active compound, Gnidilatimonoein, could have restrained effects on the expression of iNOS on the induced glial cells; however, further investigation is warranted. UR - https://www.ijbiotech.com/article_167566.html L1 - https://www.ijbiotech.com/article_167566_d10ad1a61bd421232a22e18e4aa52084.pdf ER -