TY - JOUR ID - 102188 TI - Detection of Single-Nucleotide Polymorphism in AGPAT6 Gene, Associated with Milk Fat Content, using Tetra-Primer ARMS PCR-Based Assay, in Karan Fries Breeding Bulls JO - Iranian Journal of Biotechnology JA - IJB LA - en SN - 1728-3043 AU - Upadhyay, Arpan AU - Chakravarty, Atish Kumar AU - De, Sachinandan AU - Gupta, Ashok Kumar AU - Singh, Avtar AU - Selvan, A. Sakthivel AD - Krishi Vigyan Kendra, Jhansi, Banda University of Agriculture and Technology, Banda AD - Animal Genetics and Breeding Division, ICAR-National Dairy Research Institute, Karnal- 132001, Haryana, India AD - Animal Biotechnology Division, ICAR-National Dairy Research Institute, Karnal- 132001, Haryana, India AD - Animal Genetics and Breeding Division, Tamil Nadu Veterinary and Animal Sciences University, Chennai- 600051, Tamil Nadu, India Y1 - 2019 PY - 2019 VL - 17 IS - 4 SP - 78 EP - 82 KW - AGPAT6 gene KW - Allele substitution effect KW - Karan Fries bulls KW - Milk fat content KW - SNP KW - T-ARMS PCR DO - 10.30498/ijb.2019.102188 N2 - Background: The bovine AGPAT6 gene is one of the potential candidate genes governing milk fat synthesis.Objectives: Identification of single nucleotide polymorphisms (SNP) in the targeted region of AGPAT6 gene and their effect on expected breeding values (EBV) of first lactation milk production traits viz. fat %, fat yield and 305 days milk yield in Karan Fries (KF) breeding bulls were sought. Materials and Methods: A tetra-primer ARMS PCR technique was adapted to genotype an SNP, g.36,175,805C>T located on 5’ flanking region of AGPAT6 gene. The relationship between EBV of milk production traits and polymorphic locus of AGPAT6 gene was assessed. Results: Three kinds of genotype (CC, CT, and TT) with respect to g.36,175,805C>T SNP locus were observed. The identified SNP had significant (P<0.05) influence on EBV of fat % (EBV-FP). The KF bulls with CC and CT genotype had comparatively higher EBV-FP than the bulls with the TT genotype. The substitution of “C” allele by “T” allele led to a decrease of 0.0045 % in the EBV-FP. Conclusion: The identified SNP was significantly associated with EBV-FP, thus it may be utilized as a molecular marker for developing marker-assisted selection strategy to enhance the milk fat content in KF cattle population. UR - https://www.ijbiotech.com/article_102188.html L1 - https://www.ijbiotech.com/article_102188_e18d5f47aeefdfc11ef3e1082ca8e096.pdf ER -