%0 Journal Article %T Differential Expression of Mitochondrial Manganese Superoxide Dismutase (SOD) in Triticum aestivum Exposed to Silver Nitrate and Silver Nanoparticles %J Iranian Journal of Biotechnology %I National Institute of Genetic Engineering and Biotechnology of Iran %Z 1728-3043 %A Karimi, Javad %A Mohsenzadeh, Sasan %A Niazi, Ali %A Moghadam, Ali %D 2017 %\ 12/29/2017 %V 15 %N 4 %P 284-288 %! Differential Expression of Mitochondrial Manganese Superoxide Dismutase (SOD) in Triticum aestivum Exposed to Silver Nitrate and Silver Nanoparticles %K Gene expression %K Silver nanoparticles %K Silver nitrate %K Superoxide dismutase %K Triticum aestivum %R 10.15171/ijb.1311 %X Background: The increasing use of nanoparticles (NPs) may have negative impacts on both organisms andthe environment. Objectives: The differential expression of mitochondrial manganese superoxide dismutase (MnSOD) gene in wheat in response to silver nitrate nanoparticles (AgNPs) and AgNO3 was investigated. Materials and Methods: A quantitative Real-Time RT-PCR experiment was carried out with MnSOD gene using RNAs isolated from wheat shoots treated for 0, 2, 6, 12, and 24 h with 100 mg.L-1 of either AgNO3 or AgNPs. Results: The results of this study showed that both treatments cause changes in the expression pattern of the MnSOD gene. While 2 and 6 h following the beginning of the stress, MnSOD expression was up-regulated significantly, in response to AgNO3 (1.4 and 2.8 fold, respectively), in response to AgNPs, it was up-regulated significant only after 6 h (1.6 fold), compared with the control. The gene expression, after 12 h in response to AgNO3 and AgNPs were down-regulated significantly (0.7 and 0.8 fold, respectively), and in the next 12 h , the expression appeared to be similar to the control. Conclusion: Exposure to both AgNPs and Ag ions led to a significant increase in MnSOD expression, but AgNO3 changed the MnSOD expression faster than AgNPs. Therefore, it is suggested that AgNO3 has greater penetrability and effectiveness. %U https://www.ijbiotech.com/article_54477_690e49ef7874e821a3678dbdbbeeafcb.pdf