@article { author = {Mashreghi, Mansour and Prosser, James I}, title = {Construction and Characterization of a Lux-Marked Phenanthrene Degrading Bacterium}, journal = {Iranian Journal of Biotechnology}, volume = {2}, number = {4}, pages = {243-249}, year = {2004}, publisher = {National Institute of Genetic Engineering and Biotechnology of Iran}, issn = {1728-3043}, eissn = {2322-2921}, doi = {}, abstract = {Construction of a luminescent microbial biosensor as a sensitive and rapid biotechnological tool for monitoringsurvival and activity of genetically engineered microorganisms (GEMs) in the contaminated environment hasbeen the main focus of this study. Four rifampicin resistant phenanthrene-degrading bacteria viz.,Commamonas testosteroni GZ38A, C. testosteroni GZ39, Pseudomonas putida GZ44 and P. stutzeri P16were made using gradient plate techniques. All resistant strains were transformed with the plasmid harboringthe mini-Tn5-tet transposon cassette, containing the luxAB genes to confer stability. C. testosteroniGZ38A and P. stutzeri P16 were successfully luxmarked in this manner. It was found that lux-markedstrains of C. testosteroni GZ38A were not able to degrade phenanthrene. Although the maximum growthrate of lux-marked strain was significantly lower (P < 0.05) than that of the wild type P. stutzeri P16, the P.stutzeri P16-luxAB4 was selected because it has the following criteria: the stability of expression of tetresistance over 180 generations, phenanthrene - degradability and high level of luminescence light outputin the absence and presence of phenanthrene. The addition of 1 litter (0.5 % v/v) n-decyl aldehydeproduced consistently high levels of luminescence at various stages of selected strain. Results clearly indicatedthat lux-marked strain was appropriately constructed and it is a novel biodegradative luminescentbiosensor which enables us to monitor the fate of a phenathrene bacterial degrader genetically or nongenetically made within a polluted environment.}, keywords = {Lux AB gene,Phenanthrene,Molecular Marker and Degradative bacteria}, url = {https://www.ijbiotech.com/article_6916.html}, eprint = {https://www.ijbiotech.com/article_6916_9e28f71a1e77eb471886d7558b3d789f.pdf} }