Altitudinal genetic variations among the Fagus orientalis Lipsky populations in Iran

Document Type: Research Paper

Authors

1 Research Institute of Forests and Rangelands, P.O. BOX 13185-116, Tehran, I.R. Iran.

2 Institute of Plant Genetic, CNR, Via Madonna del Piano, I-50019 Sesto Fiorentino, Firenze, Italy.

Abstract

Nuclear simple sequence repeats (nSSRs), together with 16 different enzyme loci, were used to analyze
genetic diversity and differentiation among beech (Fagus orientalis Lipsky) populations along two altitudinal
gradients in Hyrcanian forests of Iran. Both enzymes and nSSR analyses revealed a high level of
genetic diversity in natural populations of F. orientalis. The genetic diversity, estimated by expected heterozygosity, was 0.19 (by enzymes) and 0.65 (by nSSRs). Genetic variation across both markers did not reveal genetic structuring along altitudinal transects. There was less genetic variation among altitudinal gradients within transects compared to transect sites. Differentiation assays and analysis of molecular variance
(AMOVA) indicated that there was a relatively low genetic differentiation among populations, and just 1%
and 5% of the genetic variation occurred among populations by nSSR and enzyme data, respectively.
Mantel tests showed that there was not a significant correlation between the genetic distances among populations and the distance of elevation. The results of the present study indicate that the relatively low genetic differentiation among F. orientalis populations at different elevations was not caused by ecological factors. These patterns suggest that higher rates of gene flow along altitudinal gradients within transects, than
between transects; a process that could question altitudinal adaptation.

Keywords