Document Type : Research Paper
Biotechnology Group, Department of Chemical Engineering, Faculty of Engineering, Tarbiat Modares University, P.O. Box 14115-143, Tehran, I.R. Iran.
2Department of Chemical Technology, Lappeenranta University of Technology, Lappeenranta, Finland.
A flow-through biosensor consisting of a fixed bed bioreactor was employed to detect the insecticide
paraoxon. Based on the inhibition of organophosphorous insecticide to the enzymatic activity of acetylcholinesterase (AChE), using paraoxon as a model compound, the condition for detection of the insecticide were optimized. The influence of enzyme loading on the packing surface was studied and AChE loading was set at 0.36 U/cm2 for subsequent studies.
Maximum value of absorbance response occurred at the residence time in bioreactor of 5 min, that was chosen as the optimal residence time. This flow-through system gave a linear response (R2 = 0.9869) to
acetylthiocholine iodide (ATChI) at concentrations of 0.050 to 1 mM. Under appropriate conditions, the inhibition of paraoxon was proportional to its concentration in two ranges, from 0.25 to 25 mg/l and 25 to 60 mg/l and the detection limit for paraoxon was 7.3×10-8 M.
The incubation time was 14 min. These results demonstrate that silicate-multiwall carbon nanotube
(MWCNT) sol film is very efficient for retaining the activity of AChE with a good long-term stability.