Transient expression of human growth hormone in potato (Solanum tuberosum), tobacco (Nicotiana tobacum) and lettuce (Lactuca sativa) leaves by agroinfiltration

Document Type: Research Paper

Authors

1 Department of Plant Molecular Biology, National Institute for Genetic Engineering and Biotechnology (NIGEB), P.O. Box: 14155-6343, Tehran, I.R. Iran

2 Department of Plant Molecular Biology, National Institute for Genetic Engineering and Biotechnology (NIGEB), P.O. Box: 14155-6343, Tehran, I.R. Iran.

3 Department of Bioprocess Engineering, National Institute for Genetic Engineering and Biotechnology (NIGEB), P.O. Box: 14155-6343, Tehran, I.R. Iran

Abstract

Using agro-infiltration technique, we have transiently expressed human Growth Hormone (hGH) in tobacco
(Nicotiana tobacum), potato (Solanum tuberosum) and lettuce (Lactuca sativa) leaves. Out of three different
inoculation times used for infiltration in our study, it was seen that highest level of hGH expression was
achieved when leaves were infiltrated for 35 min. The presence of biologically active hGH was detected in
leaf extract by western blotting and ELISA. The highest expression was measured in tobacco was found to
be1.5-3 hGH mg/kg leaves.

Keywords