Micropropagation of Phyla nodiflora (L.) Greene: An important medicinal plant

Document Type: Research Paper

Authors

1 Department of Plant Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli-620 024, Tamil Nadu, India.

2 Department of Biotechnology, School of Life Sciences, Bharathidasan University, Tiruchirappalli-620 024, Tamil Nadu, India.

Abstract

Nodal explants of Phyla nodiflora (L.) Greene, were cultured on Murashige and Skoog (MS) medium supplemented with different combination and concentration of cytokinins and auxins for multiple shoots regeneration. The maximum numbers of shoots were found in MS medium supplemented with
2.5 mgl-16-benzylaminopurine (BA) and 0.5 mgl-1 kinetin (KN). Elongated shoots were transferred to rooting medium containing quarter strength or half strength or full strength MS medium supplemented with Indole acetic acid (IAA) or Indole-3-butyric acid (IBA). Highly efficient roots were promoted on half strength MS medium supplemented with 1.0 mgl-1Indole-3-butyric acid (IBA). Rooted plantlets were successfully
acclimatized and established in soil. This protocol could be very useful for mass cultivation of phyla nodiflora
(L.). In present study, we have established higher frequency of shoot regeneration from nodal explants of phyla nodiflora (L.).

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