Evaluation of genetic variability in a breeder flock of native chicken based on randomly amplified polymorphic DNA markers

Document Type: Research Paper

Authors

1 Laboratory for Molecular Genetics and Animal Biotechnology, Department of Animal Science, Sari College of Agricultural Science, Mazandaran university, Sari, I.R. Iran.

2 Laboratory for Molecular Genetics and Animal Biotechnology, Department of Animal Science, Sari College of Agricultural Science, Mazandaran University, Sari, I.R. Iran.

3 Department of Animal Science, College of Agricultural Science, Tehran University, Karaj, I.R. Iran.

4 Research Center of Jehad-e-Kesahavarzi, Esfahan, I.R. Iran.

Abstract

A study was undertaken to evaluate the genetic variation in the 10th generation of a breeder flock of native breed selected for high egg and meat production in native fowls breeding station, Mazandaran, Iran. Venous blood samples were collected from 100 birds of both sexes. The RAPD-PCR technique was applied to generate a DNA fingerprint of individuals. Initially, a total of 20 ten-nucleotide arbitrary primers were used but 14 of 20 primers revealed a pattern with scorable amplified bands. From a total number of 140 scored bands 63 (45%) and 77 (55%) were described as polymorphic and monophormic respectively. The average number of bands per primer varied from 4 to 16 and with sizes varying from 200 to 2100 bp in length. The estimated band sharing frequency varied from 0.79 to 0.96 between individuals. The average genetic similarity and genetic variance between individuals within the population were 0.89 and 0.11, respectively. The existence of high levels of polymorphism after 10th generation of selection may indicate the accuracy of used the selection program and also the large enough effective population size in this breeding flock. It could be concluded
that RAPD markers are effective in detecting genetic similarities and genetic variances among individuals in poultry breeder flocks.

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