1
Department of Biotechnology, Pasteur Institute of Iran, Tehran, I.R. Iran.
2
INSERM U636, University of Nice-Sophia Antipolis, France.
Abstract
Expression of foreign proteins in mammalian milk is becoming a widespread strategy for high-level production of recombinant pharmaceuticals, especially those with the most complex post-translational modifications. A gene construct was generated, consisting of 10.7 kbp of the ovine beta-lactoglobulin (oBLG) gene including its promoter and 3´ flanking region with the calcitonin coding sequences inserted in-frame into the oBLG fifth exon. The gene construct was purified using CsCl gradient, released from vector, and gel-purified. It was microinjected into fertilized mouse oocytes. These oocytes were then transferred to pseudo-pregnant foster mice. The pups born from foster mice were genotyped using PCR, slot blot, and Southern blot techniques. Among 9 mice which showed positive PCR results, only 6 mice transmitted the transgene to the next generation. Therefore, 6 transgenic lines were established which stably transmitted their transgene to their progeny.
Niavarani, A., Zeinali, S., Karimi, M., & Rassoulzadegan, M. (2004). Development of Transgenic Mice Harboring Ovine Beta Lactoglobulin-Calcitonin Transgene. Iranian Journal of Biotechnology, 2(3), 158-163.
Niavarani, A., Zeinali, S., Karimi, M., Rassoulzadegan, M. (2004). 'Development of Transgenic Mice Harboring Ovine Beta Lactoglobulin-Calcitonin Transgene', Iranian Journal of Biotechnology, 2(3), pp. 158-163.
VANCOUVER
Niavarani, A., Zeinali, S., Karimi, M., Rassoulzadegan, M. Development of Transgenic Mice Harboring Ovine Beta Lactoglobulin-Calcitonin Transgene. Iranian Journal of Biotechnology, 2004; 2(3): 158-163.
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