Effect of Kinetin on Multiple Shoot Induction in Cotton (Gossypium hirsutum L.) cv. NIAB-999

Document Type: Brief Report

Authors

1 Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad-Pakistan.

2 Cotton Research Institute, AARI, Jhang Road Faisalabad-38060, Pakistan.

3 Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad-Pakistan. 2Cotton Research Institute, AARI, Jhang Road Faisalabad-38060, Pakistan.

Abstract

Multiple shoot induction was studied in upland cotton cv. NIAB-999. Cotyledonary nodes obtained from
aseptically raised seedling were cultured on modified Murashige and Skoog medium (MS) supplemented
with different doses of Kinetin. Cotyledonary nodes produced maximum number of shoots (3.43 shoots/
explant) when cultured on MS medium supplemented with 0.25 mgl-1 Kinetin. Highest percentage (93.3 %) of
root development and root length (5.85 cm) was obtained when shoots were cultured on MS medium
supplemented with 0.5 mgl-1 napthalene acetic acid (NAA) and 0.1 mgl-1 Kinetin.

Keywords