National Institute for Genetic Engineering and Biotechnology (NIGEB), Tehran, I.R.Iran.
Department of Pathology, Cancer Institute of Tehran, Tehran University of Medical Sciences, Tehran, I.R.Iran.
Department of Surgery, Tehran University of Medical Sciences, Tehran, I.R.Iran.
Genetic Clinic Vallieasr square, Tehran, I.R. Iran.
NAD(P)H: quinone oxidoreductase (NQO1) plays an important role in detoxification of numerous endogenous
and foreign compounds. This gene has a single nucleotide polymorphism at site of codon 187
(CCT TCT). Recently, it has been demonstrated that individuals with T allele may exhibit resistance to
quinone based anticancer drugs such as mitomycin C. In the present study, a simple and feasible method was
developed for detection of NQO1 genotype. In this modified procedure, dimethylsulfoxide (DMSO) and
Triton X-100 were eliminated, also, PCR cycling conditions were modified to improve the PCR products from
blood and formalin-fixed, paraffin-embedded tissues. PCR-RFLP and DNA sequencing analysis carried out
on a limited number of blood and archival samples. It is suggested that this procedure convenient for NQO1