Department of Biochemistry, National Research Institute for Genetic Engineering and Biotechnology, Tehran, I.R. Iran.
A simple preparative method was developed for purification of Tyrosinase from edible mushroom (Agaricus
bispora). A homogenized extract of mushroom was first saturated by ammonium sulfate. The desired precipitate was mixed thoroughly with DEAE-Cellulose (DE-52) and washed out to produce melanin free precipitate. The obtained protein solution was dialyzed against running water for 4 hrs, then, concentrated and
chromatographed on a DE-52 column. On the basis of the activities assay, the eluted fractions by 150 mM
salt solution were selected for further purification. The collected fractions were pooled and chromatographed
on a Sephadex G-200 column. Polyacrylamide gel electrophoresis (PAGE) of the purified tyrosinase produced
a single band right beside the commercial sample obtained from Sigma Company at 128 kDa. The
lyophilized form of the purified Tyrosinase had a purification degree of 104 and showed strong cresolase
and catecholase activities when compared to a commerically available tyrosinase.