Department of Biology, Faculty of Science, Imam Hossain University, Tehran, I.R. Iran.
Department of Biology, Faculty of Science, Tehran University, Tehran, I.R. Iran.
Department of Biology, College of Basic Science, Shahed University, Tehran, I.R. Iran.
Botulinum neurotoxins constitute a family of bacterial toxins for botulism syndrome in human. The toxins
bind with high affinity to nerve cells where they cause a complete inhibition and release of neurotransmitters
and thereby produce flaccid paralysis. In this study the binding domain of type E neurotoxin was isolated by
PCR and expressed in a proper expression vector. The results of this investigation can be used as a tool
to study the mechanism of binding of holotoxins. This study is also implicated in antibody production against