Frequency of Bovine Lymphocyte Antigen DRB3.2 Alleles in Sarabi Cows

Document Type: Research Paper


1 Department of Animal Science, Agriculture Faculty, Ferdowsi University, Mashhad, I.R. Iran.

2 Jahad-Keshavarzi Research Center, Mashhad, I.R. Iran.

3 Department of Animal Science, Agriculture Faculty, Tehran University, Tehran, I.R. Iran.


The second exon of the bovine Major Histocompatibility Complex (MHC) class II DRB3 gene was amplified
by polymerase chain reaction (PCR) from 50 DNA samples of Iranian Sarabi cattle. Bovine DNA was isolated
from aliquots of whole blood. A two-step polymerase chain reaction followed by digestion with
restriction endonucleases RsaI, BstyI, and HaeIII was conducted on DNA from samples. Fifteen Bovine
Lymphocyte Antigen (BoLA)-DRB3 alleles were assigned, including some that were only recently
described for zebu cattle. Allelic frequencies ranged from 0.02 to 0.23. The most frequent alleles were *52
(frequency = 0.23), *11 (0.18) and *23 (0.15). Results of this study demonstrate that the BoLA-DRB3.2 locus
is highly polymorphic in Sarabi cattles.