Ralstonia eutropha accumulates poly(β-hydroxybutyrate) up to 80% of its dry weight (as a carbon and energy source) and is the best-known PHB producer. Although PHB has many potential applications in medicine, veterinary practice, agriculture and surgery, its high cost limits the widespread use of it. The recent researches have focused on reducing these costs by optimizing fermentation process. In this study the Placket Burman experimental design was used to test the relative importance of medium components and process variables on cell growth and PHB production. The optimum values of the variables, selected as the best conditions for further studies in the development of a low cost and effective fermentation process for PHB production, were as follows: initial fructose concentration, 15 g/l; C/N ratio, 7.4; shaking rate, 200 rpm; fermentation time, 40 h; temperature, 30°C; seed age, 15 h .
Khosravi Darani, K., Vasheghani Farahani, E., & Shojaosadati, S. A. (2003). Application of the Plackett-Burman Statistical Design to Optimize Poly(β-hydroxybutyrate) Production by Ralstonia eutropha in Batch Culture. Iranian Journal of Biotechnology, 1(3), 155-161.
MLA
Kianoosh Khosravi Darani; Ebrahim Vasheghani Farahani; S. Abbas Shojaosadati. "Application of the Plackett-Burman Statistical Design to Optimize Poly(β-hydroxybutyrate) Production by Ralstonia eutropha in Batch Culture". Iranian Journal of Biotechnology, 1, 3, 2003, 155-161.
HARVARD
Khosravi Darani, K., Vasheghani Farahani, E., Shojaosadati, S. A. (2003). 'Application of the Plackett-Burman Statistical Design to Optimize Poly(β-hydroxybutyrate) Production by Ralstonia eutropha in Batch Culture', Iranian Journal of Biotechnology, 1(3), pp. 155-161.
VANCOUVER
Khosravi Darani, K., Vasheghani Farahani, E., Shojaosadati, S. A. Application of the Plackett-Burman Statistical Design to Optimize Poly(β-hydroxybutyrate) Production by Ralstonia eutropha in Batch Culture. Iranian Journal of Biotechnology, 2003; 1(3): 155-161.