Growth and Isolation of Human Cytomegalovirus on a New Human Fetal Foreskin Fibroblast-derived Cell Line in Iran

Document Type: Brief Report

Authors

1 Virology Department, School of medical Sciences, Tarbiat Modarres University, P.O. Box: 14115-111, Tehran, Iran.

2 Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran.

3 3National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, I.R. Iran.

4 Biotechnology Department, Pasteur Institute of Iran, Tehran,l Iran.

Abstract

Cell culture technique has been used for detection and confirmation of many different viruses in clinical samples. Although, new diagnostic methods have been developed for viral infections, traditional cell culture
technique is still regarded as the “gold standard” for several infectious agents such as human cytomegalovirus
(HCMV). In the present study, a new human fetal foreskin fibroblast (HFFF)-derived cell line was obtained from a normal Iranian male fetus at the end of first trimester at National Cell Bank of Iran (NCBI) and its ability for HCMV growth was investigated.
Thirteen treated urine samples from renal transplant recipients were inoculated onto HFFF monolayer.
HCMV growth was monitored and confirmed by typical CMV cytopathic effect (CPE), histological staining,
immunological staining and polymerase chain reaction (PCR)-based restriction fragment length polymorphism
(RFLP) method and sequencing. All of the abovementioned
detection methods were confirmed that new
HFFF-derived cell line is a sensitive line for growth of
HCMV. This cell line has been named HFFF-PI6 and is
available with accession number NCBI C170 at NCBI
of Pasteur Institute of Iran.

Keywords