(2003). Isolation and Genetic Fingerprinting of Pseudomonas aeruginosa from Iranian Patients with Cystic Fibrosis Using RAPD-PCR. Iranian Journal of Biotechnology, 1(2), 95-100.
. "Isolation and Genetic Fingerprinting of Pseudomonas aeruginosa from Iranian Patients with Cystic Fibrosis Using RAPD-PCR". Iranian Journal of Biotechnology, 1, 2, 2003, 95-100.
(2003). 'Isolation and Genetic Fingerprinting of Pseudomonas aeruginosa from Iranian Patients with Cystic Fibrosis Using RAPD-PCR', Iranian Journal of Biotechnology, 1(2), pp. 95-100.
Isolation and Genetic Fingerprinting of Pseudomonas aeruginosa from Iranian Patients with Cystic Fibrosis Using RAPD-PCR. Iranian Journal of Biotechnology, 2003; 1(2): 95-100.
Isolation and Genetic Fingerprinting of Pseudomonas aeruginosa from Iranian Patients with Cystic Fibrosis Using RAPD-PCR
Sixty four Iranian patients with cystic fibrosis (CF) were studied for colonization with Pseudomonas aeruginosa. The patient’s age ranged between 2 months to 18 years old. Twenty one patients were colonized, 15 with non-mucoid and 6 with mucoid strains of P. aeruginosa. The colonization rate increased with age and the mucoid phenotype was only recovered from the older patients. All mucoid strains came from patients with respiratory disease whereas most of the non-mucoid isolates (n=13) were from patients with gastrointestinal disorder. The antibiograms of the isolates showed 100% sensitivity to Imipenem and Collistin followed by Ciprofloxacin (90.5%), Ceftazidime, and Tobramycin (85.7%), Amikacin, Piperacillin and Tazobactam-Piperacillin (81%), Ticarcillin (76%), Gentamycin (62%), Mezlocillin (52.4%) and Carbenicillin (43%). The MICs for Ceftazidime, Gentamycin and Tobramycin agreed with the disk test results. However, MIC determination for Amikacin showed a 100% sensitivity compared to the disk test where 81% sensitivity was observed. The discrepancy may be due to the fact that over 20% of the isolates had borderline MIC values for Amikacin. The genomic fingerprinting of the 21 isolates as well as the non-mucoid revertants ofthe mucoid strains was carried out by RAPD-PCR using primer 272 which was previously used for typing P. aeruginosa isolates from CF patient’s. Thirteen genotypes were found among the 21 isolates. One fingerprint (A) was found in 6 patients and another (B) was shared by 2 patients, all from the same health center. The idea of the hospital as environmental source or cross infection between patients cannot be ruled out.
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