Department of Biology, Faculty of Science, Yazd University, Yazd, Iran.
DOI:10.15171/ijb.1307
Abstract
Background: The transversion of G to T (G894T) in human endothelial nitric oxide synthase (eNOS) gene has profound effects such as male infertility, recurrent miscarriage, multiple sclerosis and cardiovascular diseases. Objectives: Development of a new Multiplex Tetra-Primer Amplifi cation Refractory Mutation System - Polymerase Chain Reaction (T-ARMS-PCR) for detection of rs1799983 (G894T) in the human eNOS was sought. Materials and Methods: A T-ARMS-PCR for rs1799983 polymorphism in a single-step PCR was carried out, and the results were confi rmed by PCR-RFLP technique in 82 infertile men with varicocele. Results: The results showed that GG (varicocele infertile men), GT and TT genotypes appear to be 53.65%, 34.14%, and 12.19%, respectively. Full accordance between PCR-RFLP and T-ARMS-PCR methods for genotyping of rs1799983 polymorphism was found. Conclusions: This is the fi rst work that describes a rapid, relatively cheap, high throughput detection of G894T polymorphism in eNOS that can be used in large scale clinical studies.
Heidari, M. M., & Khatami, M. (2017). Designing and Validation of One-Step T-ARMS-PCR for Genotyping the eNOS rs1799983 SNP. Iranian Journal of Biotechnology, 15(3), 208-212. doi: DOI:10.15171/ijb.1307
MLA
Mohammad Mehdi Heidari; Mehri Khatami. "Designing and Validation of One-Step T-ARMS-PCR for Genotyping the eNOS rs1799983 SNP". Iranian Journal of Biotechnology, 15, 3, 2017, 208-212. doi: DOI:10.15171/ijb.1307
HARVARD
Heidari, M. M., Khatami, M. (2017). 'Designing and Validation of One-Step T-ARMS-PCR for Genotyping the eNOS rs1799983 SNP', Iranian Journal of Biotechnology, 15(3), pp. 208-212. doi: DOI:10.15171/ijb.1307
VANCOUVER
Heidari, M. M., Khatami, M. Designing and Validation of One-Step T-ARMS-PCR for Genotyping the eNOS rs1799983 SNP. Iranian Journal of Biotechnology, 2017; 15(3): 208-212. doi: DOI:10.15171/ijb.1307
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