Optimization of Dendrimer Polyamidoamin Electrospun Nanofibers: Preparation and Properties

Document Type : Research Paper

Authors

1 Department of Polymer and Textile Engineering, Yazd Branch, Islamic Azad University, Yazd, Iran

2 Department of Textile and Polymer Engineering, Yazd Branch, Islamic Azad University, Yazd, Iran

3 Department of Polymer and Textile Engineering, Yazd Branch, Islamic Azad University, Yazd, Iran/ 2Department of Textile, Campus de Azurém, Av. da Universidade, 4800-058 Guimarães, Portugal

Abstract

Background: The most common polymers in the treatment of wounds are natural (e.g., polysaccharides, proteins, and peptides) and synthetic polymers (e.g., poly-glycolic acid, polyacrylic acid, polylactic acid, and polyvinyl alcohol) due to their biodegradability, biocompatibility, and their structural resemblance to the macromolecules known to the human body.
Objectives: The current study aimed to develop an electrospinning method using the nanofibers of polyvinyl alcohol (PVA)/carboxymethyl cellulose (CMC)/polyamide amine (PAMAM)/tetracycline (Tet) to cover the wound. The antibacterial effect of PAMAM was also tested against E. coli and S. aureus bacteria.
Materials and Methods: The morphology of the composite nanofiber was studied by a field emission scanning electron microscope. Infrared spectroscopy (FTIR) was used to characterize the nano chemical structure.
Results: Nanofibers were evaluated based on the release of different amounts of the antibiotic tetracycline (1%, 3%, 5%, and 7% by weight) while preventing wound infection. The findings indicated that the highest-profile release of all nanofibers occurred early within 12 hours. It was found that nanofiber membranes loaded with 1%, 3%, and 5% tetracycline released drugs for over 28 days, while those containing 7% tetracycline released drugs for more than 14 days.
Conclusions: According to the findings related to the drug release of PVA/CMC/15% PAMAM/Tet and surface morphology of the nanofibers, the optimal amount of Tet was 5%. The results of FTIR spectroscopy indicated that the tetracycline and polyamidoamine were successfully placed in nanofibers.

Keywords

Main Subjects